Words similar to peroxidase
Example sentences for: peroxidase
How can you use “peroxidase” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first proposes that the mutated SOD1 interacts with NO to produce peroxynitrite (ONOO -), which damages proteins by modifying tyrosine to nitrotyrosine residues [ 42 43 44 ] . The second postulates that the mutant SOD1 possesses an enhanced peroxidase activity, which causes oxidative modification of nucleic acids, proteins and lipids [ 45 46 47 ] . Both of these activities require copper at the active center of SOD1.
The DNA fragments were detected immunologically by the ELISA technique using an anti-DNA-antibody coated microplate to capture the DNA fragments, and an anti-BrdU-antibody peroxidase conjugate to detect the BrDU contained in the captured DNA fragments.
Blots were washed and incubated with secondary antibody (1:1500 horseradish peroxidase) conjugated antimouse (eNOS) or antirabbit (nNOS, iNOS, and β-actin) for 45 min at 37°C.
Next, the Tyramide Signal Amplification (TSA™) was then used to amplify the Cy-3 and Cy-5 signals using antibody-enzyme conjugates, α-DNP-Horseradish peroxidase (HRP) and α-Streptavidin-HRP with Tyramide linked Cy-3 and Cy-5.
Membranes were then probed with antibodies at ten-fold higher dilution than used for immunofluorescence for one hour, followed by one hour incubation with peroxidase conjugated secondary antibodies and detection with chemiluminescence.