Words similar to peroxidase
Example sentences for: peroxidase
How can you use “peroxidase” in a sentence? Here are some example sentences to help you improve your vocabulary:
After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.
After several washings with PBS, secondary antibodies conjugated with streptavidin/horseradish peroxidase were added for 30 min-1 hour.
Tissue was washed and treated with 0.5% hydrogen peroxide to remove endogenous peroxidase activity.
After several washes in PBS (room temperature), the sections were incubated with peroxidase labeled secondary antibody - goat anti-mouse IgG and IgM, pre-absorbed with human serum (Jackson Immunoresearch, West Grove, PA), diluted 1:50 and absorbed with rat kidney homogenate as described [ 39 ] . Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.
Dual-color immunohistochemistry [ 35] experiments were performed by using two unlabeled primary antibodies and the combined peroxidase/fluorescein isothiocyanate (FITC) technique.
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