Example sentences for: peroxidase-coupled

How can you use “peroxidase-coupled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After extensive washing, the slides were incubated with biotinated donkey anti-goat polyclonal antibody (DAKO) pre-absorbed with rat kidney homogenate [ 15 ] , followed by peroxidase-coupled streptavidin, and the peroxidase visualized by diaminobenzidine as recommended by the kit (LSAB) vendor (DAKO).

  • The membrane was incubated overnight at 4°C on a shaker, washed 4 × 15 minutes with PBS at room temperature (RT), incubated with a horseradish peroxidase-coupled anti-mouse antibody (1:5000, Dianova, Hamburg) for 3 h at and then washed again 4 × 15 minutes with PBS at RT.

  • After extensive washes in PBS, the slides were incubated for 20 minutes with mouse anti-human cytokeratin 18, clone DC10 (DAKO Corporation, Carpinteria, CA) diluted 1:50 and peroxidase-coupled secondary antibody as above.

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