Words similar to peroxidase-conjugated

Example sentences for: peroxidase-conjugated

How can you use “peroxidase-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.
Membranes were probed with alkaline phosphatase-conjugated IgG (Santa Cruz, 1:1500) or horseradish peroxidase-conjugated IgG (Santa Cruz, 1:2000).
Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.
1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.
In the aforementioned study, an ELISA assay was used to indirectly calculate affinity by incubating receptor-bound toxin with a peroxidase-conjugated anti-Cry1Aa antibody over 1.5 hours at 37°C.