Words similar to peroxidase-conjugated
Example sentences for: peroxidase-conjugated
How can you use “peroxidase-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Nitrocellulose membranes were then incubated with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody for 1 hour and washed as above.
The primary antibody was monoclonal mouse anti-hemagglutinin (dilution 1:1000 in PBS-T, gift of Guojon Bu, Washington University, St. Louis), and the secondary antisera was horseradish peroxidase-conjugated sheep anti-mouse IgG (dilution 1:1000 in PBS-T, Amersham, Arlington, IL).
Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.
Primary antibodies were detected with peroxidase-conjugated anti-mouse or rabbit IgG secondary antibodies (KPL, Gaithersburg, MD) diluted 1:2000.
This was done by visualization of the first unlabeled antibody (HLA-DR) by the peroxidase-conjugated antibody as above [ 32], without hematoxylin counterstain.