Example sentences for: peroxidase-conjugated

How can you use “peroxidase-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After incubation with horseradish peroxidase-conjugated secondary antibodies, the binding of antibodies was detected using the ECL Western Blotting System (Amersham Pharmacia Biotech, Piscataway, NJ).

  • The blots were washed (3 × 15 min) in TBS-T and were incubated for 2 hours with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, 1:5000, Pierce).

  • This was followed by incubation with a 1:2000 dilution of goat anti-mouse or sheep anti-rabbit IgG horse-radish peroxidase-conjugated antibody (Transduction Laboratories and New England Biolabs) for 1 hour at room temperature.

  • The sections were incubated in horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse immunoglobulins for 30 min and developed with 3,3'-diaminobenzidine (DAB) Chromogen solution (DAKO).

  • Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.

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