Example sentences for: peroxidase-conjugated

How can you use “peroxidase-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Primary antibodies were detected with peroxidase-conjugated anti-mouse or rabbit IgG secondary antibodies (KPL, Gaithersburg, MD) diluted 1:2000.

  • Membranes were probed with alkaline phosphatase-conjugated IgG (Santa Cruz, 1:1500) or horseradish peroxidase-conjugated IgG (Santa Cruz, 1:2000).

  • Membranes were then incubated with a secondary antibody, horseradish peroxidase-conjugated anti-rabbit IgG (1:4000, ICN Biomedical Research Products, Costa Mesa, CA, USA).

  • Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.

  • Nitrocellulose membranes were then incubated with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody for 1 hour and washed as above.

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