Example sentences for: peroxidase-conjugated

How can you use “peroxidase-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Primary antibodies were detected with peroxidase-conjugated anti-mouse or rabbit IgG secondary antibodies (KPL, Gaithersburg, MD) diluted 1:2000.

  • The blots were washed (3 × 15 min) in TBS-T and were incubated for 2 hours with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, 1:5000, Pierce).

  • This was done by visualization of the first unlabeled antibody (HLA-DR) by the peroxidase-conjugated antibody as above [ 32], without hematoxylin counterstain.

  • A biotinylated second antibody was applied for 10 min, followed by incubation with horseradish peroxidase-conjugated streptavidin for 10 min.

  • Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.


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