Words similar to pegfp-c

Example sentences for: pegfp-c

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To generate Rab24 fusions with Enhanced Green Fluorescent Protein (EGFP), cDNAs encoding full-length Rab24(wt) or Rab24(D123I), or the Rab24 C-terminal hypervariable domain (residues 166-203) were generated with flanking KpnI and BamHI sites and subcloned into pEGFP-C1 (Clontech Inc., Palo Alto, CA) so that the Rab inserts were in-frame with a 5' EGFP coding sequence.
The PCR fragment was digested with BglII and HindIII and subcloned into the correspondingly digested pEGFP-C1 (Clontech).
DNA encoding the full-length human cPLA 2 was cloned into the vector pEGFP-C3 (Clontech) to create pEGFP-cPLA 2 , as previously described [ 15 ] . The XbaI/PstI fragment from a cPLA 2 α clone containing S505A or S727A mutations [ 13 ] was inserted into an XbaI/PstI site in pEGFP-cPLA 2 to generate pEGFP-cPLA 2 S505A and pECFP-cPLA 2 S727A.