Words similar to pegfp-n

Example sentences for: pegfp-n

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The coding regions were tagged with a C -terminal green fluorescent protein (GFP) sequence by subcloning into the EcoR 1 site of the pEGFP-N1 vector (Clontech, Palo Alto, CA).
Murine L929 cells were transfected with the constructed Hyal-1-pEGFP-N1 or Hyal-2-pEGFP-N1 vector, or a control pEGFP-N1 vector by electroporation (40 μg DNA/ 5 × 10 6cells; 250 volt and 960 Fd), as previously described [ 10 ] . The cells were cultured in the continuous presence of G418 (200 g/ml) for approximately 2 weeks, and the resulting total cell colonies were harvested.
pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
pEGFP-N280 was digested with EcoRI and BamHI, filled in with Klenow DNA polymerase on both ends, and re-ligated to generate pEGFP-N200.
α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.