Words similar to pegfp-n

Example sentences for: pegfp-n

How can you use “pegfp-n” in a sentence? Here are some example sentences to help you improve your vocabulary:

Murine L929 cells were transfected with the constructed Hyal-1-pEGFP-N1 or Hyal-2-pEGFP-N1 vector, or a control pEGFP-N1 vector by electroporation (40 μg DNA/ 5 × 10 6cells; 250 volt and 960 Fd), as previously described [ 10 ] . The cells were cultured in the continuous presence of G418 (200 g/ml) for approximately 2 weeks, and the resulting total cell colonies were harvested.
005 μg μl -1; pEGFP-N1; Clontech) to facilitate later identification of successfully injected cells.
pEGFP-N346 ΔRINGwas obtained by first introducing two XhoI sites into the construct, subsequently digesting with XhoI, and then re-ligating.
pEGFP-N280 was digested with EcoRI and BamHI, filled in with Klenow DNA polymerase on both ends, and re-ligated to generate pEGFP-N200.
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.