Words similar to pel
Example sentences for: pel
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The precise biological role of SLS in streptococcal infections remains controversial [ 7 ] . The original analysis of the sagA gene demonstrated that inactivation of the gene encoding the polypeptide component of SLS rendered the organism less virulent in a dermonecrotic mouse model [ 3 ] . In a related series of studies, Li et al also isolated a mutant that not only lacked SLS activity but also affected other phenotypes [ 4 ] . The additional phenotypes included surface M and M-related protein as well as the secreted cysteine protease, streptococcal pyrogenic exotoxin B, SpeB [ 4 ] . The disrupted gene was termed pel (pleotrophic effect locus).
To determine if selection for a phenotypic variant was also occurring with the sagA/pel mutant, mice were injected in a skin air sac and recovered at varying times post infection from either the spleen, liver or kidney.
Surprisingly, at the conclusion of this study there was no statistically significant difference (p > 0.05) between the mice injected with the wild type isolate and the sagA/pel mutant (data not shown).
Arabidopsis thaliana (ecotype Columbia) plants were transformed by dipping the influorescence of flowering plants into transformation solution (5% (w/v) sucrose and 0.05% (v/v) Silwet L-77) with Agrobacterium tumefaciens strain GV3101/MP90 transformed with pEL700 or pEL702.
RNA was prepared from wild type CS101 wt, CS101 sagA/pel ::Tn 917 and CS101 sagA/pel ::Tn 917 kidney-recovered (KR) variants grown overnight (37°C 10% CO 2 ) in 40 ml THY media.
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