Words similar to pel
Example sentences for: pel
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The kidney-recovered mutant not only recovered fibrinogen binding potential, that was lost when the sagA/pel gene was inactivated, but also the level of fibrinogen-binding exceeded that of the wild type isolate (Fig.
To determine whether the β-hemolytic positive sagA/pel variants recovered from the kidney of an infected mouse maintained the Tn 917 transposon in the original location, we performed XL-PCR and Southern blot analysis as well as sequencing the region near the Tn 917 insertion using chromosomal DNA from the parental sagA/pel strain and the kidney-recovered isolates as templates.
Surprisingly, at the conclusion of this study there was no statistically significant difference (p > 0.05) between the mice injected with the wild type isolate and the sagA/pel mutant (data not shown).
The precise biological role of SLS in streptococcal infections remains controversial [ 7 ] . The original analysis of the sagA gene demonstrated that inactivation of the gene encoding the polypeptide component of SLS rendered the organism less virulent in a dermonecrotic mouse model [ 3 ] . In a related series of studies, Li et al also isolated a mutant that not only lacked SLS activity but also affected other phenotypes [ 4 ] . The additional phenotypes included surface M and M-related protein as well as the secreted cysteine protease, streptococcal pyrogenic exotoxin B, SpeB [ 4 ] . The disrupted gene was termed pel (pleotrophic effect locus).
This is due, in part, to the assembly requirement of a carrier molecule, e.g. double stranded RNA, and a peptide to form the functional hemolysin [ 2 ] . Recent genetic and immunochemical studies have clearly identified the sagA/pel gene as being responsible for the key peptide component of SLS [ 3 4 5 6 ] .
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