Words similar to pel
Example sentences for: pel
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This transcript was not seen in the sagA/pel mutant or kidney-recovered variants grown under these conditions (Fig.
Previous studies have demonstrated that the inactivation of the sagA/pel gene product also effects expression of other key streptococcal products, including surface M proteins, streptokinase (SK) and the secreted cysteine protease, SpeB [ 4 ] . Analysis of SpeB, SK and M and M-related proteins was conducted to determine if restoration of the expression of the large sagA/pel transcript also reverted the other phenotypes associated with the wild type organism.
In isolate CS101 the pel gene acted as a transcriptional regulator [ 4 ] while in an M6 isolate it displayed effects on secretion and membrane anchoring [ 8 ] . The transposon inserted in the pel gene mapped to the promoter region of the previously identified sagA gene (SLS-associated gene A).
The 5'-end of the sagA/pel mRNA was determined by the extension of the 5'-end 32P-labeled oligonucleotides 5'-ACCTTATTTTAAAAATAAAGTTAA-3' following the method of Sambrook [ 45 ] . Oligonucleotides were labeled with [γ- 32P] ATP (10 mCi/mL in aqueous solution) (Amersham, Arlington Heights, IL) and T4 polynucleotide kinase (Gibco BRL Life Technologies, Rockville, MD).
The insertion site for the Tn 917 transposon is in the promoter region of the sagA/pel gene and no sagA/pel message was detected in the original mutant [ 4 ] . RNA was isolated from the β-hemolytic positive kidney-recovered variant and the wild type isolate and analyzed for sagA/pel message by Northern blotting, (Fig.
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