Words similar to pel
Example sentences for: pel
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To determine whether the β-hemolytic positive sagA/pel variants recovered from the kidney of an infected mouse maintained the Tn 917 transposon in the original location, we performed XL-PCR and Southern blot analysis as well as sequencing the region near the Tn 917 insertion using chromosomal DNA from the parental sagA/pel strain and the kidney-recovered isolates as templates.
The precise biological role of SLS in streptococcal infections remains controversial [ 7 ] . The original analysis of the sagA gene demonstrated that inactivation of the gene encoding the polypeptide component of SLS rendered the organism less virulent in a dermonecrotic mouse model [ 3 ] . In a related series of studies, Li et al also isolated a mutant that not only lacked SLS activity but also affected other phenotypes [ 4 ] . The additional phenotypes included surface M and M-related protein as well as the secreted cysteine protease, streptococcal pyrogenic exotoxin B, SpeB [ 4 ] . The disrupted gene was termed pel (pleotrophic effect locus).
1 kb) [ 25] was then inserted into pEL700 and the resulting vector designated pEL702.
Direct genomic sequencing of the sagA/pel ::Tn 917 insertion junction in these kidney-recovered β-hemolysis positive variants established that the transposon was present in the genome in exactly the same location as the parental β-hemolytic negative sagA/pel mutant.
To test this possibility, two groups of six mice were injected with 2 × 10 9cfu of either wild type or an isogenic sagA/pel mutant isolate and the mice observed over a 72 hour period.
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