Words similar to pet-

Example sentences for: pet-

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To express the putative PfPP5 protein, we subcloned it in the bacterial expression vector pET-15b and introduced the plasmids into E. coli BL21(DE3).
3, when induced with IPTG, the pET-15b-PfPP5 clone produced a polypeptide of approximate Mr 70 k (lane 2), which is in excellent agreement with the calculated MW of 69,139, considering that pET-15b adds a (His) 6 tag (about 2 kDa) to the N-terminus of the recombinant protein.
3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).
His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).
The recombinant protein was expressed from pET-15b clone and purified by Ni-chelation chromatography (Fig.