Words similar to pet-

Example sentences for: pet-

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Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.
His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).
The recombinant protein was expressed from pET-15b clone and purified by Ni-chelation chromatography (Fig.
The HIV-1RT expression clones (pKK223-3 RT66 and pET-28a-RT51) constructed in this laboratory [ 29 30 31 ] were used for PCR amplification and construction of the insertion mutants in the p66 and p51 subunits of HIV-1 RT.
After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.