Words similar to pet-

Example sentences for: pet-

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The expression vector pET-28a and E. coli expression strain BL21 (DE3) were obtained from Novagen.
To express the putative PfPP5 protein, we subcloned it in the bacterial expression vector pET-15b and introduced the plasmids into E. coli BL21(DE3).
The (His) 6 -tagged PfPP1 expressed from pET-15b-PfPP1 was purified through Ni +2-chelation chromatography [ 14 ] as described by the manufacturer (Novagen), with 1 mM MnCl 2 being present in all the buffers.
The HIV-1RT expression clones (pKK223-3 RT66 and pET-28a-RT51) constructed in this laboratory [ 29 30 31 ] were used for PCR amplification and construction of the insertion mutants in the p66 and p51 subunits of HIV-1 RT.
A fragment containing the PPT1 ORF was excised from the pBII-PPT1 using Eco RI and Xho I restriction enzymes and was subcloned downstream of the GST coding region into the Eco RI and Sal I sites of the bacterial expression vector pET-21a GST [ 37 ] . To construct the pET-GST-PPT1 (1-504) plasmid a 453 bp fragment was amplified from pET-GST-PPT1 by PCR using a 3' primer containing a Xho I restriction site and a stop codon designed to truncate the PPT1 gene product at Met504 (5'-gatcctcgagTTAcattggttttatatctggg) and a 5' primer (5'-gtaatgcatggtggttta) encompassing the Nsi I restriction site of PPT1 . The PCR product was cloned into the Nsi I and Xho I restriction sites of pET-GST-PPT1 and sequenced.