Example sentences for: pet-

How can you use “pet-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).

  • The gene encoding the T. acidophilum A-ATPase A subunit was cloned into the expression vector pET-11a (Stratagene) and transformed into E. coli Bl21(DE3) and E. coli Bl21-CodonPlus(DE3)-RIL strain for protein expression.

  • After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.

  • The HIV-1RT expression clones (pKK223-3 RT66 and pET-28a-RT51) constructed in this laboratory [ 29 30 31 ] were used for PCR amplification and construction of the insertion mutants in the p66 and p51 subunits of HIV-1 RT.

  • A fragment containing the PPT1 ORF was excised from the pBII-PPT1 using Eco RI and Xho I restriction enzymes and was subcloned downstream of the GST coding region into the Eco RI and Sal I sites of the bacterial expression vector pET-21a GST [ 37 ] . To construct the pET-GST-PPT1 (1-504) plasmid a 453 bp fragment was amplified from pET-GST-PPT1 by PCR using a 3' primer containing a Xho I restriction site and a stop codon designed to truncate the PPT1 gene product at Met504 (5'-gatcctcgagTTAcattggttttatatctggg) and a 5' primer (5'-gtaatgcatggtggttta) encompassing the Nsi I restriction site of PPT1 . The PCR product was cloned into the Nsi I and Xho I restriction sites of pET-GST-PPT1 and sequenced.


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