Words similar to pet-
Example sentences for: pet-
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To express the putative PfPP5 protein, we subcloned it in the bacterial expression vector pET-15b and introduced the plasmids into E. coli BL21(DE3).
3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).
The gene encoding the T. acidophilum A-ATPase A subunit was cloned into the expression vector pET-11a (Stratagene) and transformed into E. coli Bl21(DE3) and E. coli Bl21-CodonPlus(DE3)-RIL strain for protein expression.
To construct a pBS58 ftsZ mutant plasmid (pBS58 ftsZ mut), the EcoRI and BstEII fragment of ftsZ in pBS58 was replaced with the same part of ftsZ from pET- ftsZ mut.
His-tagged P protein was expressed by IPTG-mediated induction of a pET-15b-RSV P (Long) clone in E. coli BL21(DE3).
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