Words similar to pet-

Example sentences for: pet-

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To construct a pBS58 ftsZ mutant plasmid (pBS58 ftsZ mut), the EcoRI and BstEII fragment of ftsZ in pBS58 was replaced with the same part of ftsZ from pET- ftsZ mut.
To express the putative PfPP5 protein, we subcloned it in the bacterial expression vector pET-15b and introduced the plasmids into E. coli BL21(DE3).
The (His) 6 -tagged PfPP1 expressed from pET-15b-PfPP1 was purified through Ni +2-chelation chromatography [ 14 ] as described by the manufacturer (Novagen), with 1 mM MnCl 2 being present in all the buffers.
Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.
3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).