Words similar to pevrf-ets-

Example sentences for: pevrf-ets-

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We then performed electrophoretic mobility shift assays (EMSA) using radiolabelled Ets oligonucleotide with nuclear extracts prepared from pEVRF-ets-1 transfected M12 cells (figure 3a, lane 3-9).
The pEVRF-ets-1 transfected M12 nuclear extracts form an additional protein:DNA complex (complex 1, figure 3, lane 3) compared with the nuclear extracts from M12 cells transfected with the control plasmid, pEVRF0 (figure 3, lane 2).
The Ets-1 expression plasmid, pEVRF-ets-1, contains the parental expression plasmid pEVRF0 and the insertion of the Ets-1 cDNA.
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
For the following experiments we used an Ets-1 expression plasmid (pEVRF-ets-1) which encodes Ets-1 protein [ 33, 34].