Words similar to pfa

Example sentences for: pfa

How can you use “pfa” in a sentence? Here are some example sentences to help you improve your vocabulary:

Although extraction can be done before, during, or after fixation, we used simultaneous extraction and fixation, because (i) cytoskeletal molecules may be rearranged or altered during fixation, as irreversible crosslinking of polypeptides is not complete even after 15 min incubation with 4% PFA at 37°C; (ii) although actin was more difficult to extract, moesin and other polypeptides were removed already with 0.5% DOTMAC in the absence of PFA.
DOTMAC/PFA was superior to TCA and the standard Triton X-100 permeabilization procedure to preserve microextensions and for moesin staining (Figure 4, G, K, and 4N,O,P).
Moesin staining, on the other hand, was primarily at the edge of cells and in microextensions, when cells were fixed with TCA or DOTMAC/PFA (Figure 4Gand 4K).
Therefore, formaldehyde and PFA are essentially indistinguishable.
Cells on glass coverslips were rinsed with PBS(+), fixed and/or extracted by one of the procedures listed in Table 1. Methanol-free formaldehyde solution can be obtained from commercial sources, but it has to be used within a week and is more expensive than PFA powder [ 61].