Words similar to pfa

Example sentences for: pfa

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The DOTMAC/PFA method provided excellent morphological preservation and immunolabeling with high fluorescence intensity of actin stress fibers and microextensions (Figure 3Qand 3S).
Our studies provide first systematic data that cells should be kept in 4% PFA for at least 60 min at room temperature or for 20 min at 37°C to irreversibly crosslink most of the polypeptides (Figure 6).
5 % DOTMAC in PBS(+) on ice for 5 min, followed by fixation with 1.0% PFA in PBS on ice for 20 min (DOTMAC/PFA method), preserved not only micropodia (white arrow, Figure 2, Eand 2F), but also microvilli (black arrow, Figure 2Eand 2F), and ultramicroextensions (white arrowhead, Figure 2F).
Although phalloidin staining and immunolabeling of tubulin were poor in cells prepared by the DOTMAC/PFA method (Figure 3Rand 3T), this method provided the widest spectrum of reactivity with the probes investigated here (Figure 3and Table 1).
Incubation with sodium borohydride after fixation in PFA had no effect either (data not shown) suggesting that reversible bridges may have formed that could not be reduced.