Words similar to pfa

Example sentences for: pfa

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Figure 5demonstrates another example of NIH3T3 cells stained with actin monoclonal and moesin polyclonal antibodies after DOTMAC/PFA fixation.
She finds fault with the local requirement that a custody order must be established within 30 days after a PFA is filed.
Although formaldehyde and PFA were used in papers as listed in Table 1 and formaldehyde solutions may have been obtained from commercial sources, for our study a formaldehyde aqueous stock solution was prepared by dissolving 20 g of PFA powder in 90 ml of double distilled water.
Our studies provide first systematic data that cells should be kept in 4% PFA for at least 60 min at room temperature or for 20 min at 37°C to irreversibly crosslink most of the polypeptides (Figure 6).
Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.