Words similar to pegfp

Example sentences for: pegfp

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The GFP-Siah1a-CT construct was made by subcloning the 3' end of Siah1a (from base 228) into pEGFP C1 in frame.
To generate pEGFP-COP1, pKS-COP1 was digested with ApaI and XbaI and the resulting fragment containing the MmCOP1 cDNA was subcloned into the same sites of the pEGFP I vector.
The mGluR5a-myc (pRK5; from P. F. Worley) has been described previously [ 24 ] . mGluR1a-GFP was constructed by inserting the mGluR1a cDNA into the pEGFP N1 vector (Clontech Laboratories, Palo Alto, CA).
Figure 3A( upper ) illustrates a cell injected with cDNA encoding mGluR5a-myc alone (and coexpressing pEGFP as a positive marker for expression).
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.