Words similar to pegfp

Example sentences for: pegfp

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pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
The mGluR5a-myc (pRK5; from P. F. Worley) has been described previously [ 24 ] . mGluR1a-GFP was constructed by inserting the mGluR1a cDNA into the pEGFP N1 vector (Clontech Laboratories, Palo Alto, CA).
To generate pEGFP-COP1, pKS-COP1 was digested with ApaI and XbaI and the resulting fragment containing the MmCOP1 cDNA was subcloned into the same sites of the pEGFP I vector.
Figure 3A( upper ) illustrates a cell injected with cDNA encoding mGluR5a-myc alone (and coexpressing pEGFP as a positive marker for expression).
The previously identified genomic BDNF clone [ 27 ] was digested with HindIII and HindIII fragments subcloned into the HindIII site of pEGFP.