Words similar to pecfp-cpla
Example sentences for: pecfp-cpla
How can you use “pecfp-cpla” in a sentence? Here are some example sentences to help you improve your vocabulary:
DNA encoding the full-length human cPLA 2 was cloned into the vector pEGFP-C3 (Clontech) to create pEGFP-cPLA 2 , as previously described [ 15 ] . The XbaI/PstI fragment from a cPLA 2 α clone containing S505A or S727A mutations [ 13 ] was inserted into an XbaI/PstI site in pEGFP-cPLA 2 to generate pEGFP-cPLA 2 S505A and pECFP-cPLA 2 S727A.
EYFP-cPLA 2 /ECFP-cPLA 2 S505A- or pECFP-cPLA 2 S727A-transfected MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with and incubated in Hank's balanced salt solution (HBSS) additionally buffered with 25 mM HEPES pH 7.4 (HHBSS).