Words similar to pcr
Example sentences for: pcr
How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:
The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.
We decided to verify the microarray results using samples from more patients by employing the use of other methods such as PCR, Northern blot and RNase protection assay.
Oligonucleotides (50 μM, except where indicated otherwise) and PCR amplicons (100-200 nM) in 50% DMSO were printed onto SuperAmine slides (TeleChem International, Sunnyvale, CA) using an Intelligent Automation Systems (IAS) arrayer (Cambridge, MA) with a 12-pen print head [ 16].
When-in house sequencing was done to verify nonsynonymous changes found in the in silico alignment, a minimum of two forward reads and two reverse reads was run for each sample of PCR products.
Average C t values from triplicate PCR reactions were normalized to average C t values for GAPDH RNA from the same cDNA preparations.