Example sentences for: pcr

How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To confirm that transfected, G418 resistant P19 cells expressed IDO antisense transcripts, we isolated total RNA from G418 resistant clones, reverse transcribed it into cDNA using a sense primer then PCR amplified the cDNA.

  • The initial volume of the PCR product (50 μl) was diluted to a total volume of 220 μl with hybridization buffer.

  • The C598T mutation removed a Pvu II site, thus permitting detection of the alteration by restriction digest analysis of a PCR product generated from genomic DNA.

  • Genes from the mouse testis-expressed gene list were procured as both clones and PCR products from a single supplier (RGI).

  • Similarly, OCP ligation/ERCA can be used to accurately genotype PCR products in a biallelic format, where the probes for both alleles are present in a single reaction [ 3 ] . The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] . ERCA probes have also been variously described as 'padlock probes' [ 6 7 ] or 'circularizable probes' [ 4 ] . Although OCP ligation/ERCA can be used to genotype PCR products, it also provides a means for directly genotyping genomic DNA without requiring an initial amplification of the genomic DNA locus.

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