Words similar to pcr
Example sentences for: pcr
How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR products were separated on 2.0% agarose gels and stained with ethidium bromide (Fig.
Real-Time PCR
The SSH procedure developed by Diatchenko et al . [ 33 ] has the additional advantage that it exploits the suppression PCR effect, eliminating the need for physical separation of single- and double-stranded cDNAs [ 34 ] . We have cloned and sequenced cDNA fragments representing 1,058 stress-induced genes from eight different SSH cDNA libraries.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).
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