Example sentences for: pcr

How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR was performed using template DNA from 11 Bacillus isolates that vary in relatedness to B. anthracis with primer sets designed to amplify DNA fragments from 47 different pXO2 ORFs.

  • PCR amplifications were performed in a 50 μl reaction volume with 60-100 ng genomic DNA, 200 μM of each dNTP and 1.25 units PCR enzyme mix.

  • The PCR primers (Table 2) were designed to contain restriction enzyme sites for cloning of the amplicon into the pHIP vector downstream of the hsp65 promoter as well as the eight basepairs (bp) upstream of the start codon of the gene being cloned.

  • The PCR templates consisted of methanol-fixed bacteria from a broad array of Brucella strains and isolates (listed in Table 3and the Materials and Methods section).

  • PCR and processing were as described above with the exception that the 5' human MYOC deletion products were cloned into a HindIII/Bsu36I digested pcDNA3.hMYOC wild-type vector.


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