Example sentences for: pcr

How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.

  • We decided to verify the microarray results using samples from more patients by employing the use of other methods such as PCR, Northern blot and RNase protection assay.

  • Oligonucleotides (50 μM, except where indicated otherwise) and PCR amplicons (100-200 nM) in 50% DMSO were printed onto SuperAmine slides (TeleChem International, Sunnyvale, CA) using an Intelligent Automation Systems (IAS) arrayer (Cambridge, MA) with a 12-pen print head [ 16].

  • When-in house sequencing was done to verify nonsynonymous changes found in the in silico alignment, a minimum of two forward reads and two reverse reads was run for each sample of PCR products.

  • Average C t values from triplicate PCR reactions were normalized to average C t values for GAPDH RNA from the same cDNA preparations.


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