Words similar to pcr
Example sentences for: pcr
How can you use “pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:
, genomic DNA), giving rise to a circular PCR product, or polymerase colony.
For the MRP gene, after an initial denaturation step of 95°C for 4 min, 35 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 95°C for 25 s, annealing at 54°C for 30 s, and extension at 73°C for 60 s, followed by a final step at 72°C for 8 min.
Due to difficulties in amplifying the rabbit MYOC coding region from cDNA, we pieced together the entire contiguous open-reading frame using rabbit genomic DNA (Clontech; New Zealand White) as a template with a multi-step PCR/ligation process.
Finally, we experimentally validated a subset of the predictions by orientation-specific reverse transcription PCR (RT-PCR).
Primers were designed from the BAC clone (NCBI, NIH, USA, RP11-407) containing the MADS-IX sequence to obtain a 2 kb sized PCR product encompassing the MADS-IX region (Table 1).