Words similar to pcr-rflp
Example sentences for: pcr-rflp
How can you use “pcr-rflp” in a sentence? Here are some example sentences to help you improve your vocabulary:
The clinical features of the studied subjects were shown in Table 1. Using the PCR-RFLP assay, we identified 24 AA, 27 AT, and 4 TT subjects.
We first used DNA from the H1975 cell line (which contains both T790M and L858R mutations) to confirm the specificity of the PCR-RFLP assay.
We next used this PCR-RFLP assay to assess various patient samples for the presence of the specific 2369 C→T mutation corresponding to the T790M amino acid substitution.
The identified G1754T (Gly583Cys) missense mutation of the Abcg5 generates loss of a restriction site for Hae III after PCR amplification of exon 12, and this allowed a PCR-RFLP assay to further confirm the detected change (Figure 5B).
PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications.
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