Words similar to pcr-rflp
Example sentences for: pcr-rflp
How can you use “pcr-rflp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Likewise, DNA from progressive lesions from patients 2 and 3 yielded both 97- and 106-bp fragments in the PCR-RFLP assay (Figure 3B), whereas the pre-treatment specimens did not produce the 97-bp product.
We next used this PCR-RFLP assay to assess various patient samples for the presence of the specific 2369 C→T mutation corresponding to the T790M amino acid substitution.
The identified G1754T (Gly583Cys) missense mutation of the Abcg5 generates loss of a restriction site for Hae III after PCR amplification of exon 12, and this allowed a PCR-RFLP assay to further confirm the detected change (Figure 5B).
PCR reactions and restriction digests (PCR-RFLP) are optimized for each marker and performed on the PTC-100 Programmable Thermal Controller (MJ Research) outfitted with a heated lid for oil-free amplifications.
The clinical features of the studied subjects were shown in Table 1. Using the PCR-RFLP assay, we identified 24 AA, 27 AT, and 4 TT subjects.