Words similar to pcr-amplified

Example sentences for: pcr-amplified

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The membrane containing B. thuringiensis AWO6 DNA was hybridized using a [α- 32P]dCTP-labeled probe prepared from a mixture of six PCR-amplified pXO2 ORF fragments (pXO2 ORFs 6, 10, 50, 63, 72, 81).
The Alu hooks were PCR-amplified from the 3' end of a 290-bp Alu consensus sequence [ 33 ] with pairs of alu-F, alu-R primers (Table 2).
3) The gentamicin-resistance gene ( aacC1 ) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain Hpa I restriction sites), digested with Hpa I, and cloned into the Hpa I site located between the two fragments of the sigE gene in pSEKO.
We used regions of amino acid sequence conserved between the midasin genes in yeast and Schizosaccharomyces pombe to make a rough map of the gene onto the August 2001 freeze of the assembled human genome with the public domain Blat server at http://genome.ucsd.edu/index.html [ 54 55 ] . The resultant partial nucleotide sequence identified about four-fifths of the exons and was used to design the requisite PCR primers to verify all exon-intron boundaries in the gene by physical sequencing of appropriate PCR-amplified cDNA fragments.
The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.