Example sentences for: pcr-amplified

How can you use “pcr-amplified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.

  • We have determined the sequence of the human midasin gene from PCR-amplified fragments of cDNA from testis.

  • Yeast genomic DNA was isolated from transformants and PCR-amplified as described previously [ 29 ] .

  • The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.

  • Clone inserts were PCR-amplified directly from culture and purified according to a previously published protocol [ 5, 18].


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