Example sentences for: pcr-amplified

How can you use “pcr-amplified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Yeast genomic DNA was isolated from transformants and PCR-amplified as described previously [ 29 ] .

  • PCR-amplified products were purified with Gene Clean kits (BIO101, Carlsbad, CA), and eluted in 20 μl water.

  • 3) The gentamicin-resistance gene ( aacC1 ) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain Hpa I restriction sites), digested with Hpa I, and cloned into the Hpa I site located between the two fragments of the sigE gene in pSEKO.

  • Probes were made from PCR-amplified fragments of selected clones using the ReadyPrime random primed DNA labeling kit (Amersham-Pharmacia) with [α- 32P] (ICN Biomedicals, Irvine, CA).

  • A sequencing primer was hybridized to the single-stranded copy of the PCR-amplified p53/K- ras 2 fragment and a single base extension with either a Cy-3 or Cy-5 labeled dNTP was performed prior to scanning on a microarray scanner.


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