Example sentences for: pcr-based

How can you use “pcr-based” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For example, cDNAs that are not easily amplified by means of PCR are inadequately represented in PCR-based methods.

  • Subsequently, sequence analysis identified KRC as a member of the ZAS family of proteins which share the ability to bind κB-like motifs [ 15 ] . DNA competition analysis showed that KRC fusion proteins containing the ZAS-C domain bind specifically to both the RSS and to the κB motif [ 14 28 ] . DNA footprinting analysis further showed that KRC/ZAS-C binds to specific nucleotides within the κB and the heptamer of the RSS [ 14 ] . In this study, using a PCR-based DNA-binding site-selection and amplification procedure, we demonstrated that both the N-terminal ZAS-N and the C-terminal ZAS-C domains are able to bind GT-rich DNA sequences, and confirmed that the RSS and κB motifs are the high-affinity targets of KRC.

  • To identify new Ser/Thr phosphatases of Pf, we have recently undertaken a PCR-based approach.

  • This alternative screening strategy could be particularly useful for large coding regions where a PCR-based screen might fail.

  • VP16-ERβ mutations and Gal-N-CoR mutations were prepared using standard PCR-based site directed mutagenesis (Quickchange Kit, Stratagene, La Jolla, CA) and confirmed by sequencing.


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