Words similar to pcr-based
Example sentences for: pcr-based
How can you use “pcr-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
RFLP markers and ESTs were converted to co-dominant PCR-based molecular markers (CAPS) as described by Konieczny and Ausubel [ 33].
VP16-ERβ mutations and Gal-N-CoR mutations were prepared using standard PCR-based site directed mutagenesis (Quickchange Kit, Stratagene, La Jolla, CA) and confirmed by sequencing.
However, assays using the polymerase chain reaction (PCR) have demonstrated that DNA of nontypeable H. influenzae is present in a substantial proportion of these middle ear fluids [ 15 16 17 18 19 20 ] . Furthermore, reverse transcriptase PCR-based assays have shown the presence of bacterial mRNA, indicating that bacteria are present in a viable and metabolically active but non-culturable state [ 21 ] . These observations suggest that in otitis media with effusion, bacteria are in a physiological state which differs from that of bacteria growing planktonically in a free floating phase.
For example, cDNAs that are not easily amplified by means of PCR are inadequately represented in PCR-based methods.
There are several strategies for identifying differentially expressed transcripts, including differential display (DD), representational difference analysis (RDA), serial analysis of gene expression (SAGE), enzymatic degradation subtraction and subtractive hybridization [ 29 30 31 32 33 ] . We chose the PCR-based suppression subtractive hybridization (SSH) procedure for several reasons: it includes a normalization step, it enriches for differentially expressed transcripts, and it yields cDNA fragments that can be used directly for the construction of DNA microarrays.
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