Words similar to pcr-based
Example sentences for: pcr-based
How can you use “pcr-based” in a sentence? Here are some example sentences to help you improve your vocabulary:
Using PCR-based approaches, Knight and Kendrick-Jones [ 43] cloned a myosin they called ATM ( Arabidopsis thaliana myosin), Kinkema and Schiefelbein [ 41] cloned the myosin MYA1 and Kinkema et al.
This alternative screening strategy could be particularly useful for large coding regions where a PCR-based screen might fail.
There are several strategies for identifying differentially expressed transcripts, including differential display (DD), representational difference analysis (RDA), serial analysis of gene expression (SAGE), enzymatic degradation subtraction and subtractive hybridization [ 29 30 31 32 33 ] . We chose the PCR-based suppression subtractive hybridization (SSH) procedure for several reasons: it includes a normalization step, it enriches for differentially expressed transcripts, and it yields cDNA fragments that can be used directly for the construction of DNA microarrays.
The filter collection and PCR-based detection methods developed here offers some clear advantages over the use of more traditional methods such as impinger collection and cell culture based detection.
Rad26 is a regulatory subunit of the Rad3 checkpoint kinase [ 18 ] . To investigate the cellular distribution of Rad26, we used a one step PCR-based gene-modification method [ 41 ] to fuse GFP (S65T) to the C-terminus of the genomic copy of rad26 +. The Rad26-GFP fusion protein maintained proper checkpoint function following hydroxyurea (HU) and Bleomycin treatments (data not shown).
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