Words similar to pcmv-tag

Example sentences for: pcmv-tag

How can you use “pcmv-tag” in a sentence? Here are some example sentences to help you improve your vocabulary:

To construct the expression plasmid encoding C-terminal-myc-tagged truncated BTBD2 having the N-terminal half the BTB domain deleted (m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445-2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 3 vector (Stratagene) cut with BamH I and Hind III.
To construct the expression plasmid encoding C-terminal-myc-tagged, full length BTBD2 (m-BTBD2F), the entire coding region of BTBD2 (1-1575 bp) was amplified with primers (Upper primer: 5' ATGGCGGCGGGTGGGAGCGGCGGGCGTGCGTCGTG-3', Lower primer: 5'-GGTGTAGAAGATGACCTCGGGGATCTGGCCGTCCTCCAC-3') containing BamH I and Hind III cleavage sites and inserted into pCMV-Tag 5 vector (Stratagene) cut with BamH I and Hind III.