Example sentences for: pcmv

How can you use “pcmv” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The mammalian expression constructs of Smad1 and its deletion mutant Smad1C (271-465) were made by subcloning Smad1 into two modified pCMV6 vectors, which have either the Flag epitope (F-pCMV6) or the T7 epitope (T7-pCMV6) placed upstream of an array of new multiple cloning sites.

  • The cDNAs encoding Rab24(wt) and Rab24(D123I) were subcloned into a pCMV5 vector that had been modified by PCR to encode an in-frame polyhistidine tag (His 6 ) at the amino-terminus of the Rab protein.

  • T7-ΔN3 pCMV6 was made by PCR amplification of HsN3 (a.a.

  • 45-263) followed by subcloning the PCR products into the EcoR1/Xho1 sites of the modified T7-pCMV6 vector.

  • HEK 293 cells grown in 10 cm diameter dishes were transfected with either pCMV5- His 6 Rab24 or pCMV5- His 6 Rab24(D123I) as described earlier.


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