Words similar to pcmv

Example sentences for: pcmv

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HEK 293 cells grown in 10 cm diameter dishes were transfected with either pCMV5- His 6 Rab24 or pCMV5- His 6 Rab24(D123I) as described earlier.
N3-F pCMV6 was made by PCR amplification of HsN3 (1-263) followed by subcloning the PCR products into the EcoR1/EcoRV sites of pFlag-CMV5C vector (Kodak).
The mammalian expression constructs of Smad1 and its deletion mutant Smad1C (271-465) were made by subcloning Smad1 into two modified pCMV6 vectors, which have either the Flag epitope (F-pCMV6) or the T7 epitope (T7-pCMV6) placed upstream of an array of new multiple cloning sites.
T7-ΔN3 pCMV6 was made by PCR amplification of HsN3 (a.a.
pCMV5 expression vectors containing Rab24 and Rab1B were engineered by overlap-extension PCR to encode the following chimeric proteins: Rab24/Rab1B, consisting of residues 1-165 of Rab24 [ 24 ] fused to residues 163-201 from Rab1B [ 80 ] ; Rab24(D123I)/Rab1B, consisting of residues 1-165 of Rab24 with the D123I substitution, fused to residues 163-201 of Rab1B; Rab1B/Rab24, consisting of residues 1-162 of Rab1B fused to residues 166-203 of Rab24; and Rab1B(N121I)/Rab24, consisting of residues 1-162 of Rab1B with the N121I substitution, fused to residues 166-203 of Rab24.