Example sentences for: pcr-blunt

How can you use “pcr-blunt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.

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