Words similar to ocp
Example sentences for: ocp
How can you use “ocp” in a sentence? Here are some example sentences to help you improve your vocabulary:
OCP 3' arm length and ligation reaction temperature
Indeed when these reactions were analyzed on an agarose gel, the size of the characteristic ERCA DNA ladder was different from that obtained with amplification of the OCP (data not shown).
The above experiments involved a two-tube assay with 100 ng of genomic DNA and one OCP/P2 combination per tube.
MW designed H63D and C282 probes and performed H63D OCP genotyping.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
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