Words similar to ocp
Example sentences for: ocp
How can you use “ocp” in a sentence? Here are some example sentences to help you improve your vocabulary:
One OCP annealed perfectly to the normal sequence of interest, and the other targeted the mutant.
Isothermal exponential amplification produces a fluorescent signal from ligated OCP using nanomolar amounts of probe.
As expected, when the target DNA was heterozygous, a fluorescence signal was observed with both OCP/primer combinations (Fig.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
Use of 40 nM OCP and 100 nM oligonucleotide target allowed sufficient signal for detection without the ERCA step that is required for genomic DNA target.