Words similar to ocps
Example sentences for: ocps
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Previously, it was shown that 20 ng of genomic DNA, equivalent to ∼ 6000 copies of the gene, was sufficient to detect a C/T polymorphism using the serial invasive signal amplification reaction (SISAR) [ 18 ] . In order to investigate the sensitivity of ERCA for detecting SNPs, 1 ng of genomic DNA, equivalent to ∼ 300 copies of the gene, was used as target for circle formation with OCPs for the SNP G1822A.
The OCP backbone sequence connecting the target complementary 5' and 3' regions is unique for the two allele-specific OCPs.
OCPs were designed such that the 3' terminal nucleotide annealed opposite the mutation of interest (Figure 1).
With both mutant and normal OCP in the reaction, either one or the other or both OCPs were circularized, depending on the genotype.
Sequences of oligomers are shown in Table 1. All OCPs were 5' phosphorylated and gel purified.