Words similar to nucleosides

Example sentences for: nucleosides

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To address these questions, we used an analytical method developed in our laboratory and described in a previous study [ 24 ] . Briefly, the complex cRNA is digested with P1 nuclease and calf intestinal phosphatase and applied to a high performance liquid chromatography (HPLC) column to separate the nucleosides, followed by an absorbance measurement at 260 nm.
As seen in Figure 1A, when only the unmodified NTPs are incorporated during the IVT, there is good separation of the individual nucleosides.
Furthermore, as reported earlier [ 24 ] , using the extinction coefficients and integrating the area under these peaks, there are approximately equal amounts of each of the four nucleosides.
The enzyme was incubated with the cRNA at 55°C for 6 hours, then for 6 hours at 37°C in the presence of 10 units of calf intestine alkaline phosphatase to generate the nucleosides.