Words similar to nf-h
Example sentences for: nf-h
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Thus, it appears that SMI-31 recognizes a phosphoepitope that occurs on proteins in addition to NF-H and NF-M, as previously reported (see the Discussion for other reports of SMI-31 staining glial cells in situ ). RT-97 also labeled nuclear and cytoplasmic structures in F98 cells (Figure 1E).
Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
The RT-97 mAB [ 9 ] has been characterized as recognizing phosphorylated epitopes on the 210 kDa NF-H peptide [ 15 ] , and used similarly to SMI-31 to identify neurites in vitro and in situ [ 16 17 18 ] . One would predict, therefore, that labeling with RT97 would produce staining patterns, including nuclear, similar to those of SMI-31 in SH-SY5Y cells.
0 http://www.cbs.dtu.dk/databases/PhosphoBase/, a Web based analysis tool provided by the Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark [ 24 ] . The five amino acid sequences were aligned to NF-H using the Match-Box server located on the Internet at http://www.fundp.ac.be/sciences/biologie/bms/matchbox_submit.html.
In support of this, a search for phosphorylation motifs among NF-H, GFAP and lamins A, B, and C revealed a single, high probability tyrosine site that occurs once on each of these proteins (Figure 3), further suggesting the possibility that this site constitutes or is contained within the epitope recognized by these two antibodies.
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