Words similar to nf-h
Example sentences for: nf-h
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Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
In support of this, a search for phosphorylation motifs among NF-H, GFAP and lamins A, B, and C revealed a single, high probability tyrosine site that occurs once on each of these proteins (Figure 3), further suggesting the possibility that this site constitutes or is contained within the epitope recognized by these two antibodies.
Therefore it is possible that this sequence constitutes or contains the epitope recognized by the SMI-31 and RT-97 mABs, and may account for the cross-reactivity of these antibodies with respect to NF-H, GFAP, and lamins.
Numerous putative phosphorylation motifs were identified in the amino acid sequences of NF-H, GFAP and nuclear lamins A, B, and C when analyzed by the NetPhos server, including a single tyrosine kinase motif, LNDRFAG
Amino acid sequences for human NF-H (Accession QFHUH), lamin A/C (Accession P02545), and B (Accession P20700), and human GFAP (Accession XP_050159) were obtained from the National Center for Biotechnology Information Protein Data Base http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?db=Protein.
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