Words similar to nf-h
Example sentences for: nf-h
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In support of this, a search for phosphorylation motifs among NF-H, GFAP and lamins A, B, and C revealed a single, high probability tyrosine site that occurs once on each of these proteins (Figure 3), further suggesting the possibility that this site constitutes or is contained within the epitope recognized by these two antibodies.
Amino acid sequences for human NF-H (Accession QFHUH), lamin A/C (Accession P02545), and B (Accession P20700), and human GFAP (Accession XP_050159) were obtained from the National Center for Biotechnology Information Protein Data Base http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?db=Protein.
0 http://www.cbs.dtu.dk/databases/PhosphoBase/, a Web based analysis tool provided by the Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark [ 24 ] . The five amino acid sequences were aligned to NF-H using the Match-Box server located on the Internet at http://www.fundp.ac.be/sciences/biologie/bms/matchbox_submit.html.
Amino acid sequences predicted by PhosphoBase as serine, threonine, or tyrosine phosphorylation motifs for NF-H were then compared to the aligned proteins in regions of high identity, as determined by reliability scores generated by the Match-Box server [ 25 ] .
Additional bands, not seen in the SMI-32 lane, are present in the SMI-31 and RT-97 lanes between and above the NF-H and NF-M, and probably represent forms of NF-M and NF-H with varying degrees of phosphorylation.
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