Words similar to nf-h

Example sentences for: nf-h

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The calculated molecular weights of lamins A, B and C are approximately 74, 66, and 65 kDa, respectively; therefore, it seems most likely that lamins, NF-H, and GFAP all share a common motif that, when phosphorylated, is recognized by SMI-31 and RT-97 mABs.
Therefore it is possible that this sequence constitutes or contains the epitope recognized by the SMI-31 and RT-97 mABs, and may account for the cross-reactivity of these antibodies with respect to NF-H, GFAP, and lamins.
Additional bands, not seen in the SMI-32 lane, are present in the SMI-31 and RT-97 lanes between and above the NF-H and NF-M, and probably represent forms of NF-M and NF-H with varying degrees of phosphorylation.
Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
Amino acid sequences predicted by PhosphoBase as serine, threonine, or tyrosine phosphorylation motifs for NF-H were then compared to the aligned proteins in regions of high identity, as determined by reliability scores generated by the Match-Box server [ 25 ] .