Words similar to membranes

Example sentences for: membranes

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We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
This was accomplished by subjecting cell lysates to electrophoresis in agarose gels, followed by blotting to nitrocellulose membranes.
Proteins were transferred to PVDF membranes and analyzed with either anti-α 1S mAb IIC12 antibody [ 43], or anti-α
Membranes were hybridized for 16 h at 65°C and briefly rinsed in low-stringency buffer containing 40 mM Na 2 HPO 4 (pH 7.2), 1 mM EDTA (pH 8.0) and 5% SDS at room temperature, followed by washing in low-stringency buffer at 65°C for 30 min.
Laminin is a glycoprotein composed of three chains, A, B1, and B2 in a cross-shaped molecule; this protein is found in basement membranes where it interacts with other such components as collagen IV, heparan-sulfate proteoglycan, and nidogen [ 45 ] . Laminin contains EGF-like modules in the amino-terminal region of the A, B1, and B2 chains [ 45 ] . Surprisingly, these modules do not appear to be involved in the binding of fibulin-1.