Example sentences for: lysed

How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were again collected by centrifugation (6000 rpm × 30 min), then lysed with 20 ml of 20% sodium cholate, pH 7.1 (30 min., 4°C).

  • Cells were lysed in lysis buffer (20 mM HEPES, pH 7.4, containing 100 mM NaCl, 5 mM MgCl 2 , 5 mM EDTA, 0.5% Triton X-100 and 1 mM PMSF) with 1 × proteasome inhibitor cocktail (Boehringer Mannhein).

  • A549 cells were cultured under the condition described, and the cells were lysed and the whole cell proteins were separated on 7.5% SDS-PAGE.

  • Cells were placed on ice, washed once with PBS, then lysed in NP40 Lysis buffer as described above.

  • Cells were washed with phosphate-buffered saline and then lysed in 0.5 ml hypotonic lysis buffer by 10 passages through a 27-gauge needle.


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