Words similar to lysed
Example sentences for: lysed
How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were suspended in PBS containing the same protease inhibitors and were then lysed using a French press.
Consistent with our observation that the phosphorylation status of Rlk does not significantly contribute to its association with RAFTs, we still observed a decreased half-life of Rlk co-expressed with Fyn when lysed under these harsh conditions (data not shown), arguing that phosphorylation by Fyn specifically accelerates Rlk turnover.
All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).
After 2 hours of incubation at 37°C, cells were washed one time with PBS and subsequently lysed in 0.5 ml of lysis/wash buffer containing 1% NP40 in Tris-buffered saline plus an EDTA-free protease inhibitor cocktail (Roche; Indianapolis, USA) for 20 minutes at 4°C.
The MTT solution was then removed, and the cells in each well were lysed by the addition of 100 μl dimethly sulfoxide.