Example sentences for: lysed

How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were lysed with HMK-NP40 (50 mM Hepes, pH 7.5, 40 mM magnesium acetate, 20 mM potassium phosphate, 5% sucrose, 0.4% NP-40), clarified, and protein concentration determined as above.

  • For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.

  • Cells were lysed in a denaturing lysis buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 8.0) and then briefly sonicated in full speed.

  • After washing twice with 3 ml of plain medium, the cells were lysed with 0.2 ml of lysis buffer containing 0.1 M NaCl, 0.01 M EDTA, 0.3 M Tris-HCl (pH 8.0), 0.2 M sucrose and 0.5% SDS.

  • The colonies were lysed and the subcloned fragments were reamplified with the same primers.


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