Example sentences for: lysed

How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).

  • The remaining adherent monocytes were washed twice in PBS and simultaneously harvested and lysed for RNA isolation using 2.1 ml RNeasy lysis buffer per T175 flask, according to the manufacturer's protocol.

  • All cells were cultured to mid-log phase of growth, washed with PBS without Ca 2+and Mg 2+, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.5), 120 mM NaCl, 5 mM EDTA, 50 mM NaF, 0.2 mM Na 3 VO 4 , 1 mM DTT, 0.5% NP-40 and protease inhibitors (Protease inhibitor cocktail tablets, Boehringer Mannheim, one tablet per 50 ml).

  • Haploid segregants from three complete tetrads were cultured for 24, 48 and 72 hours in SDC medium, lysed, and levels of ApppN and AppppN were determined.

  • After washing twice with 3 ml of plain medium, the cells were lysed with 0.2 ml of lysis buffer containing 0.1 M NaCl, 0.01 M EDTA, 0.3 M Tris-HCl (pH 8.0), 0.2 M sucrose and 0.5% SDS.


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