Words similar to lysed
Example sentences for: lysed
How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were resuspended in lysis buffer (10 mM Tris (pH 7.5), 1 mM EDTA, 50 mM NaCl, 0.25% Nonidet P-40, 1 mM PMSF, 1 mg of aprotinin/mL, 0.1 mM sodium vanadate, 10 mM sodium molybdate, and 10 mM β-glycerol phosphate) and lysed by sonication with a microtip on setting 2 and 20% duty cycle for 10 s.
To prepare cell lysates, cultures were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer, and stored and processed for western blot analysis as indicated for tissue lysates above.
The cells were again collected by centrifugation (6000 rpm × 30 min), then lysed with 20 ml of 20% sodium cholate, pH 7.1 (30 min., 4°C).
The cell pellets were re-suspended in 1 ml PBS, lysed by boiling and stored as 0.1 ml aliquots at -70°C.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
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