Words similar to lysed
Example sentences for: lysed
How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total RNA was prepared directly from the frozen pellets of parasitized erythrocytes, where approximately 1 ml of cell pellet was lysed in 7.5 ml Trizol (GIBCO) and RNA was extracted according to the manufacturer's instructions.
The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).
To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.
Cells were lysed in a denaturing lysis buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 8.0) and then briefly sonicated in full speed.
Ice cold PBS was added to the cells after 2 minutes OKT3 treatment and the cells were quickly pelleted and lysed in 0.5 ml TENV (10 mM Tris 7.5, 150 mM NaCl, 5 mM EDTA, 1 mM Na 3 VO 4 ) containing 1% Triton X-100.
Loading...