Example sentences for: lysed

How can you use “lysed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was prepared directly from the frozen pellets of parasitized erythrocytes, where approximately 1 ml of cell pellet was lysed in 7.5 ml Trizol (GIBCO) and RNA was extracted according to the manufacturer's instructions.

  • The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).

  • To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.

  • Cells were lysed in a denaturing lysis buffer (8 M urea, 0.1 M NaH 2 PO 4 and 0.01 M Tris•Cl, pH 8.0) and then briefly sonicated in full speed.

  • Ice cold PBS was added to the cells after 2 minutes OKT3 treatment and the cells were quickly pelleted and lysed in 0.5 ml TENV (10 mM Tris 7.5, 150 mM NaCl, 5 mM EDTA, 1 mM Na 3 VO 4 ) containing 1% Triton X-100.


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