Words similar to lysates

Example sentences for: lysates

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In kidneys of S rats, associated with apoptosis was a temporal increase in expression of Fas, which was demonstrated using Western analysis of lysates of kidney cortex [ 7 ] . The purpose of the present study was to demonstrate the site of expression and potential functional significance of Fas and FasL in the kidneys of S rats during development of hypertensive nephrosclerosis.
After 15 min on ice, the tissue and cell lysates were sonicated using a SonicatorTM Cell Disruptor (Heat Systems-Ultrasonic, Inc., Plainview, NY) for 5 seconds, and centrifuged at 11000 × g for 20 min at 4°C.
Protein lysates from whole cells and tissues were prepared with a buffer, which contained 50 mM Tris, pH 7.5, 150 mM NaCl, 1% (v/v) Nonidet P40 (NP40), 2 mM 4-(2-aminoethyl)benzenesulphonyl fluoride, 1 μg/ml leupeptin, 2 μg/ml aprotinin and 2 μg/ml pepstatin.
We also prepared cell lysates from primary trophoblast cultures (= high p27 positive control), which were established and processed as described previously [ 18 ] .
To detect the presence of IgG antibodies to the U1-70 kD ribonuclear protein, both immunoblotting and EIA methods were used as described previously [ 16 17 18 ] . Each sample was tested by immunoblot against Jurkat cell lysates with a 1:100 dilution of sera, and by EIA against a bacterially produced U1-70 kD fusion protein that comprised residues 1-205 of u1-70 kD.