Words similar to lysates

Example sentences for: lysates

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In cases where discrepant results were obtained between immunoblot and EIA testing, sera were immunoblotted using a more sensitive technique against both intact and apoptotic Jurkat lysates, as previously described [ 18 19 ] using sera diluted 1:5000.
To make lysates, transfected cells were washed with cold HBSS, scraped, lysed on ice in 300 μl lysis buffer (20 mM Tris, PH 7.4, 150 mM NaCl, and 0.5% Triton X-100, 1mM EDTA) containing protease inhibitors.
Upon addition of 10 mM DTT to cell lysates, mAb 6B12 immunoprecipitated only a 85 kD protein band, consistent with specific recognition of the CD98 heavy chain.
After thorough washing, the cells were incubated for 1 hour at 37°C to allow in vivo ribosylation, then sonicated and lysates subjected to further C3 ribosylation by the addition of 2 μCi [ 32P]-NAD and 1 μg of C3 in ribosylation buffer.
Protein concentrations of GC lysates were determined by the bicinchoninic acid method (Micro BCA Protein Assay, Pierce, Rockford IL).