Words similar to lysates

Example sentences for: lysates

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Whole cell lysates serving as positive controls were prepared by incubating monolayers with denaturing Laemmli buffer.
RASMC lysates were subjected to partial heparitinase treatment (full digestion was not performed in order to allow some GAGs to remain associated with the core proteins) then applied to either III1-C or III 11-C Sepharose.
5 embryos, PCR was performed on lysates of extraembryonic tissue as template, using primers BGH037 (5'-ACGTTGCGAGCTGCTGCGGC-3'), BGH039 (5'-ACCCATGTCAAACCCATCAG-3'), and BGH052 (5'-CCTATGCGGACAATTCATCTG-3'), in a similar reaction mix except for 1.0-1.
Lysates (7-10 μg) from control and treated cells were separated on 10% SDS-PAGE.
Recombinant protein was purified from bacterial lysates in accordance with the published method for the QIA express system (Qiagen, Valencia, CA, USA) with variations in Buffers B and D as noted in Additional file: 1. Procedures were carried out at 4°C; dialyzed recombinant DEK protein (rDEK) was stored at -80°C.