Words similar to lysates

Example sentences for: lysates

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Blots of cell lysates were stripped and reprobed using antibodies specific for α-tubulin (1:10,000, Sigma).
Pcu3-Myc immunoprecipitated from lysates of cells expressing HA-tagged Csn1p together with Pcu3p-Myc from the endogenous promoters revealed co-precipitation of Csn1p-HA.
In an effort to explain the selective advantage of cells with a newly identified “resistance” mutation in EGFR— a T790M amino acid substitution—we further characterized the drug sensitivity of putatively resistant EGFR mutants versus wild-type or drug-sensitive EGFR mutants, using both a NSCLC cell line fortuitously found to contain the T790M mutation and lysates from cells transiently transfected with wild-type and mutant EGFR cDNAs.
Transient expression of the Src*/Cas(SD) in Cos-7 cells followed by analysis of total cell lysates using anti-phosphotyrosine antibodies demonstrated that while the attenuated Src kinase domain alone did not increase total cellular tyrosine phosphorylated proteins as expected, expression of the Src*/Cas(SD) fusion resulted in tyrosine phosphorylation of a protein of the expected size of the chimera, as well as other proteins (Fig.
After thorough washing, the cells were incubated for 1 hour at 37°C to allow in vivo ribosylation, then sonicated and lysates subjected to further C3 ribosylation by the addition of 2 μCi [ 32P]-NAD and 1 μg of C3 in ribosylation buffer.