Words similar to lysate
Example sentences for: lysate
How can you use “lysate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Centrifuged pellets of unattached cells were combined with the appropriate monolayer lysate sample and the samples were heated to 100°C for 5 min.
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
The lysate was clarified at 12,000 × g for 10 min at 4°C.
The in vivo permissiveness of the tumours was assessed by analysing the lysate of the whole tumour (without any surrounding tissue) 2 days after injection of the Ad Adapt Luc.
After inactivation of the DNase at 75°C for 5 min 5 μl of the lysate was used for first strand synthesis under the conditions recommended by the manufacturer.
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