Example sentences for: lysate

How can you use “lysate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The suspension was sonicated and the lysate centrifuged at 100,000 × g for 30 min.

  • The β-galactosidase activity in each cell lysate was measured in the 4-Methylumbelliferyl-β-D-Galactoside (MUG) assay and was used to normalize the luciferase activity analyzed in each cell lysate.

  • Equal protein amounts of each cell lysate (usually 100 ug) were incubated for 3 h at 4°C in the presence of purified GST-c-Jun-(1-79) bound to glutathione-agarose beads (2.

  • The lysate was then applied to affinity chromatography columns as described below.

  • Aliquots of phosphorylated proteins were added to a reaction containing 120 μg cell lysate, recombinant human E1, 0.25 mg/ml ubiquitin, 1 μM ubiquitin aldehyde, 1 μM okadaic acid, 20 μM MG132, and ATP regenerating system (20 mM ATP, 10 mM creatine phosphate, 0.1 μg/ml creatine kinase).


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