Words similar to lysate
Example sentences for: lysate
How can you use “lysate” in a sentence? Here are some example sentences to help you improve your vocabulary:
The lysate was transferred to a TLS55 ultracentrifuge tube, over-layered sequentially with 30% and 5% sucrose in TENV.
Parallel experiments performed using the same lysate and antibody to hemagglutinin for immunoprecipitation and western blotting show the position of 6His-HA-PP1α and the fact that endogenous PP1α is not recognized by this antibody (bottom panel).
The equivalent of 1.5 μg total lysate protein was loaded onto each lane of 10% SDS-PAGE gels, separated by electrophoresis, and transferred to nitrocellulose membranes using a Semi-Dry Trans-Blot apparatus (Bio-Rad).
Laemmli electrophoresis sample buffer (5×) was added to the lysates, and SDS-polyacrylamide gel electrophoresis and immunoblotting were performed using 35 μg lysate protein, phospho-specific antibodies for ERK and p38, and rabbit polyclonal antibody for cPLA 2 [ 21 ] .
The lysate was placed on top of buffer GF (7% Ficoll 400, w/v, 20% glycerol, 20 mM HEPES (pH = 7.2), 0.5 mM MgCl 2 ). Nuclei were pelleted by spinning at 7000 rpm in a microfuge.