Example sentences for: lysate

How can you use “lysate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The crude lysate was diluted 1:3 with Buffer A. After centrifugation (15 min at 20,000 × g at 4°C) the soluble material was loaded onto a BioRad Macroprep High Q column (2.

  • The cell lysate was allowed to bind to GST beads.

  • The lysate was placed on top of buffer GF (7% Ficoll 400, w/v, 20% glycerol, 20 mM HEPES (pH = 7.2), 0.5 mM MgCl 2 ). Nuclei were pelleted by spinning at 7000 rpm in a microfuge.

  • To determine whether SKP2 induces p27 ubiquitylation, cell lysate was prepared from MIB-exposed cells infected with Ad-SKP2 and Ad-SKP2-ΔF, and supplemented with phosphorylated p27, ATP, and ubiquitin.

  • While each of the five antisera co-precipitated at least one of the other subunits, Pip1p, Pop1p, and Pop2p antisera co-precipitated all five proteins from wild-type cell lysate (Fig.


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