Words similar to lysate
Example sentences for: lysate
How can you use “lysate” in a sentence? Here are some example sentences to help you improve your vocabulary:
The lysate was incubated on ice for 15 min, and microcentrifuged at 4°C for 10 min.
Briefly, tissues were homogenized in CHAPS buffer 10 mM (3-[3-Cholamidropropyl) dimehthylammonio]-1-propane-sulfonate, 2 mM EDTA, 4 mM iodoacetate in phosphate buffered saline (pH 7.2) with protease inhibitor cocktail (Invitrogen; Carlsbad, CA) and cleared tissue lysate prepared by centrifugation at 10,000 g for 15 minutes.
The cell lysate was mixed with 0.25 ml of Buffer B (Buffer A containing 1% NP-40 and 1% deoxycholate) and the insoluble material was removed by centrifugation at 100,000 × g for 30 min.
Radiolabeled wildtype and T178A point mutant p27 were produced by combined in vitro transcription and translation in rabbit reticulocyte lysate containing 35S-methionine.
Qiagen Ni-NTA Superflow resin was equilibrated with modified buffer B before being combined with the cell lysate.
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