Words similar to lys
Example sentences for: lys
How can you use “lys” in a sentence? Here are some example sentences to help you improve your vocabulary:
Together with Lys61, Thr59 forms one wall of each of the A-binding pockets.
The S224K mutant exhibited a 50% reduction in metal binding (Table 2), and we postulate this is due to electrostatic repulsions between the newly introduced Lys with Zn 2 . In spite of the mutants binding significant amounts of Zn(II), we included 100 μM ZnCl 2 in all of the kinetic buffers to ensure saturation of the metal binding sites and to facilitate direct comparison of the kinetic data.
Efforts to solve the crystal structure of one of the metallo-β-lactamases with a bound substrate molecule have failed, most likely due to the high activity of the enzymes towards all β-lactam containing antibiotics [ 37 54 ] . Therefore, computational studies have been used extensively to study substrate binding, the role of the Zn(II) ions in catalysis, the protonation state of the active site, and inhibitor binding [ 37 42 55 56 57 58 59 ] . All of the substrate binding models have made assumptions before the substrate was docked into the active site [ 37 42 ] , and some of these assumptions have been shown to be invalid for certain substrates [ 43 ] . With L1, two key assumptions were made: (1) the bridging hydroxide functions as the nucleophile during catalysis and (2) Zn 1 coordinates the β-lactam carbonyl [ 37 ] . With these assumptions and after energy minimizations, Ser224 was predicted to hydrogen bond to the substrate carboxylate [ 37 ] , reminiscent of the role predicted for Lys224 in CcrA [ 42 ] . Ullah et al.
Alexa 488, BODIPY Fl, fluorescein, Oregon Green 488 and tetramethylrhodamine have been conjugated to the third amino acid of SP, Lys 3. The amine group of Lys provides a convenient reactive group for labeling SP without altering its original amino acid sequence.
The lys2 marker was introduced by crossing to YCH128.
Loading...