Example sentences for: lysine-coated

How can you use “lysine-coated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.

  • Eight consecutive sections (8 μm) were cut from each ovary and mounted onto poly-L-lysine-coated glass slides.

  • The 70-bp oligonucleotides were synthesized (Operon Technologies, CA), resuspended in 3 × SSC to a final concentration of 60 pmol/μl, and spotted onto poly-L-lysine-coated microscopic slides, as previously described [ 53].

  • Following centrifugation (900 × g; 5 min) and re-suspension in fresh NCM, the cells were counted and plated onto poly-L-lysine-coated 48 well plates at a density of 0.5 × 10 6cells/well.

  • The DNA clones, in 394-well plates, were spotted onto poly-L-lysine-coated microscope slides using a robotic arrayer (Genomic Solutions, Ann Arbor, Michigan).


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