Words similar to lysing
Example sentences for: lysing
How can you use “lysing” in a sentence? Here are some example sentences to help you improve your vocabulary:
P2 was resuspended in the lysing buffer (LB, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin), and kept on ice for 15 min, homogenized and centrifuged at 19,000 g for 20 min to obtain pellet P3 and supernatant S3.
To address these possibilities, we performed similar analyses after lysing cells under harsh detergent conditions (using 1% SDS), then diluting the samples before performing immunoprecipitations.
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
Protein extracts were prepared by lysing sperm or homogenized tissues with a solubilization buffer (62.
The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).