Words similar to lysing
Example sentences for: lysing
How can you use “lysing” in a sentence? Here are some example sentences to help you improve your vocabulary:
For adenoviral transduction, lymph nodes and spleen cells were isolated, treated with ACK lysing buffer for 3 min and washed in DMEM/10 mM HEPES (pH 7.2).
Nuclear extracts were prepared by first lysing the cells for 10 minutes in 1 ml of isotonic buffer (50 mM KCl, 50 mM Tris-HCl, pH 7.9, 0.2% NP40, 0.5 mM EDTA and 1.5 mM MgCl2).
The plate was placed on a shaker for one hour at room temperature to complete the lysing process, then the optical density of each well was measured by a 96-well plate reader with a filter setting at 570 nm (reference filter setting was 630 nm).
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
To address these possibilities, we performed similar analyses after lysing cells under harsh detergent conditions (using 1% SDS), then diluting the samples before performing immunoprecipitations.