Words similar to lysing

Example sentences for: lysing

How can you use “lysing” in a sentence? Here are some example sentences to help you improve your vocabulary:

As a first step in the analysis of the sur2 clone, the time course of its survival in THP-1 macrophages was determined by infecting THP-1 macrophages, lysing the infected macrophages at various times after phagocytosis, and enumerating viable intracellular bacteria by plating on solid medium.
The plate was placed on a shaker for one hour at room temperature to complete the lysing process, then the optical density of each well was measured by a 96-well plate reader with a filter setting at 570 nm (reference filter setting was 630 nm).
Bacteriologic methods were used as previously described [ 21 22 ] . Briefly, blood samples were centrifuged 15 min at 2,000 g and leukocytes recovered by standard methods with Ficoll-Hypaque (Pharmacia Fine Chemicals, Piscataway, NJ) and then mixed with a lysing solution that contained 0.45% sodium deoxycholate.
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
P2 was resuspended in the lysing buffer (LB, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin), and kept on ice for 15 min, homogenized and centrifuged at 19,000 g for 20 min to obtain pellet P3 and supernatant S3.