Example sentences for: lysing

How can you use “lysing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nuclear extracts were prepared by first lysing the cells for 10 minutes in 1 ml of isotonic buffer (50 mM KCl, 50 mM Tris-HCl, pH 7.9, 0.2% NP40, 0.5 mM EDTA and 1.5 mM MgCl2).

  • Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).

  • Bacteriologic methods were used as previously described [ 21 22 ] . Briefly, blood samples were centrifuged 15 min at 2,000 g and leukocytes recovered by standard methods with Ficoll-Hypaque (Pharmacia Fine Chemicals, Piscataway, NJ) and then mixed with a lysing solution that contained 0.45% sodium deoxycholate.

  • The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).

  • Adherent monocytes, derived from whole blood of normal donors were purified from peripheral blood by lysing red blood cells using Red Blood Cell Lysis Solution (Sigma, St Louis, MO) and Ficoll Hypaque density separation technique.


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