Words similar to lysing
Example sentences for: lysing
How can you use “lysing” in a sentence? Here are some example sentences to help you improve your vocabulary:
P2 was resuspended in the lysing buffer (LB, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin), and kept on ice for 15 min, homogenized and centrifuged at 19,000 g for 20 min to obtain pellet P3 and supernatant S3.
Protein extracts were prepared by lysing sperm or homogenized tissues with a solubilization buffer (62.
The plate was placed on a shaker for one hour at room temperature to complete the lysing process, then the optical density of each well was measured by a 96-well plate reader with a filter setting at 570 nm (reference filter setting was 630 nm).
The cells were then washed once in staining buffer and residual red blood cells lysed with FACS' Lysing solution (Becton Dickinson).
Bacteriologic methods were used as previously described [ 21 22 ] . Briefly, blood samples were centrifuged 15 min at 2,000 g and leukocytes recovered by standard methods with Ficoll-Hypaque (Pharmacia Fine Chemicals, Piscataway, NJ) and then mixed with a lysing solution that contained 0.45% sodium deoxycholate.
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