Words similar to lysotracker

Example sentences for: lysotracker

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The effects of H 2 O 2 and O 2 •-on the fluorescence of LysoTracker Red DND-99 were studied by preparing 2 μM solutions of the dye in MES buffer solutions at pH 4.5 or 7.5, containing various concentrations of H 2 O 2 and KO 2 . After incubation at room temperature for 10 min, the emission fluorescence at λ 592 was recorded as previously described.
As shown in Figure 7, the fluorescence of the LysoTracker Red is not affected by either of these two oxygen species.
This data strongly supports the contention that decreased fluorescence of the LysoTracker Red in a subset of lysosomes does indicate elevation of pH.
The effect of pH on the fluorescence of LysoTracker Red DND-99 (final concentration is 2 μM) was similarly performed, except that the emission fluorescence at λ 592 was recorded upon excitation at λ 574 .
Cells were next washed with PBS and the fluorescence images of both oxidized OxyBURST Green H 2 HFF BSA and LysoTracker Red were recorded.