Words similar to lysyl

Example sentences for: lysyl

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To determine whether a multicopy lysyl tRNA synthetase plasmid affected accumulation of dinucleoside polyphosphates, we transformed HNT2 ADE2 strain BY71-16d and hnt2ΔADE2 strain BY71-6c with control plasmid pRS423 and with plasmid pM1.
However, deletion of lysU had no effect on heat-shock inducible AppppA accumulation [ 37 ] . The KRS1 gene [ 38 ] encoding cytosolic lysyl tRNA synthetase was cloned into multicopy plasmid pRS423 [ 39 ] to generate plasmid pM1.
The conservation of all the residues implicated in catalysis in the biochemically uncharacterized proteins such as AlkB, EGL-9, leprecan and YbiX suggests they all catalyze oxidative reactions similar to those catalyzed by IPNS, DAOCS, CAS and related enzymes such as protein lysyl/prolyl hydroxylases, EFE and leucoanthocyanidin oxidases [ 1, 2].
Additionally, to test whether moderate overexpression of the lysyl tRNA synthetase gene affected accumulation, we compared control transformants to multicopy KRS1 transformants of the two strains.
To address whether plasmid pM1 indeed increased lysyl tRNA synthetase activity, lysates from pRS423 and pM1-transformed BY71-6c were assayed for incorporation of 3H lysine into yeast tRNA.