Words similar to lcm
Example sentences for: lcm
How can you use “lcm” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sixty-eight frozen human breast tissue specimens were evaluated by LCM, qPCR and IHC, including 31 primary tumors of infiltrating ductal carcinomas (22 primary tumors with metastasis and nine primary tumors without known metastasis), 22 matched nodal metastases, 10 fibroadenomas and five normal breast tissues (obtained from reduction mammoplasty).
bp = base pairs; FISH = fluorescence in situ hybridization; LCM = laser capture microdissection; MADS = metastasis-associated DNA sequences; NCBI = National Center for Biotechnology Information; PCR = polymerase chain reaction; RDA = representational difference analysis; SCM = single cell microdissection; STS = sequence tagged sites.
We used a combination of immunohistochemistry (IHC), LCM and real-time, quantitative PCR (qPCR) analysis of material obtained from a panel of primary breast infiltrating ductal carcinomas and nodal metastases, and compared the target gene expression levels with those found in nonmalignant diseased tissue and in samples of normal breast tissue.
Having detected all three proteins immunohistochemically in normal, benign and malignant breast epithelia, the quantitation of OPN, TSP-1 and TYRP-1 mRNA was achieved using LCM and qPCR.
Unfortunately, the resolution of LCM does not enable the selection of epithelial cells from myoepithelial cells and so we were unable to confirm this quantitatively.
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