Words similar to lcm
Example sentences for: lcm
How can you use “lcm” in a sentence? Here are some example sentences to help you improve your vocabulary:
FFPE = formalin-fixed, paraffin-embedded; H&E = hematoxylin and eosin; IHC = immunohistochemistry; LCM = laser capture microdissection; OPN = osteopontin; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; qPCR = real-time, quantitative PCR; RT = reverse transcriptase; TSP-1 = thrombospondin-1; TYRP-1 = tyrosinase-related protein-1.
The total number of samples studied using LCM and qPCR were 76 (68 samples were microdissected to specifically enrich for cells of epithelial origin, and eight samples were microdissected to obtain tumor-associated stroma).
LCM was performed with the PixCell I LCM System (Arcturus Engineering, Mountain View, CA, USA).
We used a combination of immunohistochemistry (IHC), LCM and real-time, quantitative PCR (qPCR) analysis of material obtained from a panel of primary breast infiltrating ductal carcinomas and nodal metastases, and compared the target gene expression levels with those found in nonmalignant diseased tissue and in samples of normal breast tissue.
Coupled with qPCR, LCM can reveal significant differences in specific gene expression that are not accessible with relatively insensitive IHC alone.