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Example sentences for: kda

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2Ashows that the antibody to p27, clone G173-524, produces a single band at 27 kDa in all investigated tissues, and no nonspecific staining is apparent with secondary antibody alone.
Escherichia coli DNA topoisomerase I is a representative example of type IA DNA topoisomerase (for reviews, see refs [ 1 2 ] ). Its major biological role in the bacterial cell is the removal of excessive negative supercoils from DNA to maintain the DNA at optimal superhelical density along with DNA gyrase [ 3 ] . The enzyme has a molecular weight of 97 kDa and the active site tyrosine responsible for DNA cleavage is found in the 67 kDa N-terminal transesterification domain.
Specificity of the E12 and F12 monoclonal antibodies was verified by western blot analysis using baculovirus expressed form of human ERβ (53 kDa) [ 18 ] . These antibodies were also reported to identify an additional ~62-64 kDa band in protein extracts from granulosa cells of several mammalian species, in addition to the ~52 kDa band [ 18 ] .
A less prominent band was detected at ~80 kDa.
Correspondingly, in CHO.asbt lysates, an immunoreactive band of comparable size (~45 kDa) to rat asbt protein can be easily detected using an anti-asbt antibody (Figure 3B).