Words similar to kcl
Example sentences for: kcl
How can you use “kcl” in a sentence? Here are some example sentences to help you improve your vocabulary:
The binding reactions were performed in 20 μl of T buffer (50 mM Tris-HCl, pH 7.4, 50 mM KCl, 50 mM NaCl, 10 mM MgCl 2 , 0.1 mM EDTA, 0.5 mM DTT, 30 μg/ml BSA) supplemented with 5 mM ATP and 6% (v/v) glycerol for most of the reactions.
After trypsinization cells were centrifuged at 400 g for 5 min, swollen in 75 mM KCl for 10 min at 37°C, re-centrifuged and fixed in methanol:acetic acid (3:1) at 4°C, before dropping cells onto slides.
5-3 MΩ) filled with 3 M KCl.
Slices were allowed to incubate and recover for at least an hour in room temperature ACSF comprised of the following composition (mM): NaCl 117; KCl 5.4; NaHCO 3 26; MgSO 4 1.3; NaH 2 PO 4 1.2; CaCl 2 2.5; glucose 10, continuously bubbled with 95% O 2 plus 5% CO 2 .
Incorporation of tritiated lysine into tRNA was measured by modification of the protocol of Hou [ 51 ] . Reactions contained 10 micrograms of total protein in 20 mM KCl, 10 mM MgCl 2 , 4 mM dithiothreitol, 2 mM ATP, 50 mM Na HEPES pH 7.5, 20 μM lysine (10 μCi 3H lysine), and were performed with or without 15 μg yeast tRNA (Sigma) in a total volume of 60 μl.