Words similar to kcl
Example sentences for: kcl
How can you use “kcl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Pimephales promelas KCl Municipal effluent Receiving water
Cellular fractionation was performed as described in earlier reports [ 122 123 124 125 ] . Cells were scraped into 100 μl of ice-cold buffer A [10 mM Hepes (pH 7.9); 1.5 mM MgCl 2 ; 10 mM KCl; 0.5 mM DTT; 0.05% NP-40].
Adult mouse testes were homogenized in a buffer containing 20 mM Tris, pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3% NP-40, 40 U/ml of Rnasin ribonuclase inhibitor (Promega, Madison, WI), and a mixture of 10 protease inhibitors provided in the Protease Inhibitors Set (Roche Molecular Biochemicals, Indianapolis, IN).
Briefly, the first step contained 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 0.001% gelatin, 250 μM each dNTP, 4.7 μM of the forward and reverse primers, 0.15 units of Taq polymerase (Roche) in a total 5 μl reaction volume.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).