Words similar to ionomycin

Example sentences for: ionomycin

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Other investigators have shown (in mouse systems) that commitment to Th1 or Th2 cytokine production by naïve T cells requires prior cell proliferation [ 24 ] ; and reversal of Th1 or Th2-committed cells requires at least several days of proliferation [ 25 ] . When normal human PBMC were stimulated for 7 days with SEB, the presence of 5A6 anti-CD81 mAb resulted in a modest increase in IL-4-producing T cells, when those cells were restimulated with PMA and ionomycin (Figure 4A).
PBMCs (except the activation time course) and T cells were stimulated for 3 h with phorbol ester (50 ng/ml) and ionomycin (1.
After 1-7 days, cells were stimulated for 4 h with 10 ng/ml PMA + 1 μg/ml ionomycin (both from Sigma), in the presence of 10 μg/ml Brefeldin A.
Control cells were not incubated with ionomycin.
For some experiments, the transduced cells were then activated with 5 μM ionomycin and 50 ng/ml PMA for 4 h or stimulated for 18 h by immobilized anti-CD3 (pre-coated at 10 μg/ml in PBS) or 5 μg/ml OVA peptide with antigen-presenting cells before analysis.