Example sentences for: ionomycin

How can you use “ionomycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DRB, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and ionomycin were purchased from Sigma.

  • After 1-7 days, cells were stimulated for 4 h with 10 ng/ml PMA + 1 μg/ml ionomycin (both from Sigma), in the presence of 10 μg/ml Brefeldin A.

  • After transduction with Ad5.UbP.GFP or Ad5.CMV5.GFP without PMA/ionomycin, the GFP positive and the GFP negative fractions of CD4 +cells were both CD62L high and CD25 low, confirming that the cells were initially of a resting phenotype and that adenoviral transduction did not activate them.

  • Lung lymphocytes were cultured in the presence or absence of phorbol myristate acetate (PMA)/ionomycin and brefeldin A for 12 h.

  • 18 mice by in vitro culture under polarizing cytokine conditions [ 21 ] . The resulting Th1 and Th2 lines were incubated with Ad5.UbP.GFP or Ad5.CMV5.GFP, with or without activation with PMA/ionomycin, and expression of GFP was measured by flow cytometry 24 hours later (Fig.

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