Example sentences for: ionomycin

How can you use “ionomycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DRB, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and ionomycin were purchased from Sigma.

  • After 1-7 days, cells were stimulated for 4 h with 10 ng/ml PMA + 1 μg/ml ionomycin (both from Sigma), in the presence of 10 μg/ml Brefeldin A.

  • Responses are expressed as a fraction of the ionomycin (maximum) response.

  • The cells were then treated with 1 μM ionomycin for 30 minutes, after which time they were either fixed immediately or washed extensively with PBS and incubated for 6 hours in medium lacking the drug ("washout").

  • Although elevating [Ca 2+] i with ionomycin had no effect on vital dye uptake, preliminary experiments in which fibroblasts were loaded intracellularly with the Ca 2+chelator BAPTA, showed that MTX-induced responses were significantly delayed, suggesting that a rise in [Ca 2+] i is necessary, but not sufficient for MTX-induced COP formation.

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