Example sentences for: insert

How can you use “insert” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).

  • Short cytoplasmic domains (5-14 amino acids) are found at both the amino and carboxyl termini of CD9 [ 14 ] . As reported previously, we used a yeast two-hybrid system and observed interaction between the second extracellular domain of MkCD9 and the extracellular domain of proHB-EGF [ 9 ] . However, despite repeated attempts, using the yeast two-hybrid system, we were unable to show interaction between the amino terminal cytoplasmic domain of monkey CD9 and the cytoplasmic domain of proHB-EGF; these results may reflect the limitation of the yeast two-hybrid system as the insert sizes cloned into the vectors may be too small resulting in Gal4 fusion proteins whose "test subunits" are masked by the Gal4 subunit.

  • The insert of Dex1aDS was sequenced and its orientation determined by PCR using either primer ex1aP3 or sc1P18 paired with a primer (Dex1aDSP1) located within Dex1aDS.

  • Insert four (4) AA batteries into my battery compartment.

  • In contrast, only the construct carrying the p27 Kip15'-UTR insert in the correct orientation expressed a significant level of luciferase.

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