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Example sentences for: inserts

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All plasmid inserts were amplified in 100 μl reactions using the following primer sequences:
Appropriate pairs of gene and element primers were used to detect the presence or absence of the respective retrotransposon inserts associated with each gene in strains representing 12 geographically dispersed populations of D. melanogaster . The results presented in Figure 2and Table 1demonstrate that while the Burdock insertion located just 3' to cathD gene is not present in any of 12 strains representing a geographically diverse sampling of natural populations, the Antonia LTR fragment located in the intron of the heterochromatic Cht3 gene is fixed in all 12 strains tested.
Transwell inserts (24 mm diameter, 3 μm pore size) were purchased from Corning Corp. (Acton, MA).
Partial cDNA inserts and empty vectors represent a substantial part of cDNA libraries that would hamper the analysis of restriction fingerprints in standard cloning vectors; e.g. the empty cloning vector itself would generate a characteristic restriction fingerprint that would overlay and disturb every analysis.
Cosmid libraries frequently contain clones without DNA inserts [ 24 ] , so it is not too surprising that clones with small DNA fragments were present in the E. coli library after infection with λ phage.