Example sentences for: inserting

How can you use “inserting” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1/His-p35-C/EBPβ was constructed by inserting an 1,739 bp EcoRI fragment from pRSETB-EFII into an EcoRI cut pcDNA3.

  • Hence, a product called "fireplace gas valve safety covers," whose purpose was too obscure for me to understand, and the VCR Lock, which, according to the packaging, "prevents child from inserting objects into cassette opening."

  • N-terminal deletions, in addition to the series obtained through two-hybrid screening, were constructed by amplifying the two-hybrid cDNA clones using primers containing Sma I and EcoR I at the N- and C-terminus, respectively, and inserting them into both pPC86 and pGEX-2TK vectors.

  • A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (ori S ) SmaI fragment [ 5 ] . The plasmid pTK1 contains HSV-1 SC16 sequence coordinates 45,055-48,634 subcloned into the HindIII / EcoRI site of pUC19 (Invitrogen).

  • This is consistent with previous reports of Tn 5 preferentially inserting into particular locations in the target sequence [ 17 18 ] . Since these "hotspots" could become a limiting factor in the number of unique insertions recovered within a target sequence, the second reading frame through the Tn 5 MEs was used for the ECFP transposon.


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