Example sentences for: immunoprecipitations

How can you use “immunoprecipitations” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell lysates for small scale immunoprecipitations were prepared by disrupting cells in immunoprecipitation buffer (20 mM Tris/HC1, pH7.

  • For immunoprecipitations, labeled cellular proteins were extracted with lysis buffer (1% of the indicated detergent, 20 mM Hepes, pH 7.4, 150 mM NaCl, 2 mM PMSF, 10 μg/ml leupeptin, 10 μg/ml aprotinin, 2 mM MgCl2, 0.5 mM CaCl2) for 60 minutes at 4°C.

  • Immunoprecipitations from the transfected cell lysates were performed with anti-FLAG antibody coupled Agarose beads (Sigma) and then the beads were washed six times in the lysis buffer.

  • EV-M provided PP1-isoform specific antibodies and participated in PP1 immunoprecipitations and western blots.

  • For co-immunoprecipitation of p27 Kip1with CDK4 or CDK2, immunoprecipitations were performed as described above with either anti-CDK4 or anti-CDK2.


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