Example sentences for: immunoprecipitations

How can you use “immunoprecipitations” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell lysates for small scale immunoprecipitations were prepared by disrupting cells in immunoprecipitation buffer (20 mM Tris/HC1, pH7.

  • For immunoprecipitations, labeled cellular proteins were extracted with lysis buffer (1% of the indicated detergent, 20 mM Hepes, pH 7.4, 150 mM NaCl, 2 mM PMSF, 10 μg/ml leupeptin, 10 μg/ml aprotinin, 2 mM MgCl2, 0.5 mM CaCl2) for 60 minutes at 4°C.

  • Kinase activity was not detectable for the K430E mutant of Btk:ER (Fig 1B), comparable to control immunoprecipitations from Btk-deficient cells (data not shown).

  • These proteins are unlikely to include Lyn or Syk, the two PTKs that are primarily responsible for the BCR-induced tyrosine phosphorylation pattern seen in DT40 whole cell lysates [ 14], since we were unable to detect phosphorylation of Lyn and Syk following specific immunoprecipitations (data not shown).

  • EV-M provided PP1-isoform specific antibodies and participated in PP1 immunoprecipitations and western blots.

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