Example sentences for: immunostaining

How can you use “immunostaining” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After centrifugation (11000 × g for 10 min at 4°C), the supernatant was diluted to 10 ml with blocking reagent and used for immunostaining of nitrocellulose membranes.

  • Our previous studies on animal models have shown that intercalated duct cells in adult rat submandibular glands are the common progenitor for both the acinar and granular duct cells [ 27 36 ] . A recent study in human breast has revealed that CK5-positive cells are capable of giving rise to both glandular and ME cell lineages [ 21 ] . In both cases the intermediate cells showed an unusual immunostaining pattern for several proteins [ 21 36 ] .

  • But, the positive immunostaining for p53, iNOS and VEGF was not observed in normal gastric epithelial tissue and CSG.

  • Synovial tissue components including lining cells, macrophages, lymphocytes, fibroblasts, smooth muscle cells and endothelial cells were graded for immunostaining by a frequency of staining scale, scored 0-100%, where 0% indicates no staining and 100% indicates that all cells were immunoreactive.

  • Our findings are consistent with those of a recent study showing that a vast majority of the ME cells in both normal and ductal carcinoma in situ displayed distinct immunostaining to p63, SM-MHC, and calponin, whereas a single or a cluster of a few ME cells in some ducts failed to show immunoreactivity to all these three markers [ 28 ] . However, our study differs from this study [ 28 ] and previous studies [ 3 4 ] in four aspects: first, the SMA-negative cells were segmented, accounting for at least one-third or all of the ME cells in involved ducts; second, we tested for more ME cell markers; third, the SMA-negative cells assessed were morphologically similar to their adjacent SMA-positive neighbors on H&E-stained sections; fourth, our focus is directed toward the elucidation of the detailed immunohistochemical profile of these SMA-negative cells identified in 3 of 175 examined cases.


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