Words similar to immunoprecipitated
Example sentences for: immunoprecipitated
How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Radiolabeled mycRab24 was immunoprecipitated and the 32P-labeled guanine nucleotides eluted from the protein were subjected to thin-layer chromatography to resolve GDP and GTP.
10 μl of immunoprecipitated protein were loaded per sample.
To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.
Next, 30 μl of 2X SDS-sample buffer was added to elute immunoprecipitated proteins.