Example sentences for: immunoprecipitated

How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Radiolabeled mycRab24 was immunoprecipitated and the 32P-labeled guanine nucleotides eluted from the protein were subjected to thin-layer chromatography to resolve GDP and GTP.

  • 10 μl of immunoprecipitated protein were loaded per sample.

  • To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.

  • Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.

  • Next, 30 μl of 2X SDS-sample buffer was added to elute immunoprecipitated proteins.


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