Example sentences for: immunoprecipitated

How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • GluR1 was immunoprecipitated with anti-GluR1 antibody and then subjected to in vitro phosphorylation (Figure 4C).

  • For ubiquitylation reactions, Pcu3p-Myc complexes were immunoprecipitated from 100 - 200 ug total cell lysates prepared as described above.

  • Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.

  • Cytoskeletally anchored α V β 6 was immunoprecipitated after TGFβ 1 stimulation (10 nM for 10 minutes) followed by Western analysis with antibodies against tyrosine-phosphorylated proteins (C) or Western blotting after biotinylation of all proteins and streptavidin detection (D).

  • Insulin receptor was immunoprecipitated with specific antibodies, collected with protein A sepharose, and incubated with polyGlu 4 Tyr 1 peptides (Sigma, St. Louis, MO) in the presence of [γ- 32P]-ATP [ 14 ] . After completion of the kinase reaction, the mix was centrifuged and the phospho-peptide-containing supernatant precipitated with 20% TCA (w/v).


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