Example sentences for: immunoprecipitated

How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In fig 4, GSK3β kinase activity was monitored by immunoprecipitating GSK3β and then measuring the ability of the immunoprecipitated protein to phosphorylate a specific GSK3β peptide substrate.

  • Radiolabeled mycRab24 was immunoprecipitated and the 32P-labeled guanine nucleotides eluted from the protein were subjected to thin-layer chromatography to resolve GDP and GTP.

  • Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.

  • α i2 and sα i2 were immunoprecipitated from cell extracts, and incorporation of radio-label was analyzed by SDS-PAGE followed by fluorography (Figure 6A).

  • To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.


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