Words similar to immunoprecipitated

Example sentences for: immunoprecipitated

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2, expression of the S77A mutant resulted in decreased relative phosphorylation of immunoprecipitated RARα in stable clones compared to G418 resistant control cells.
Accordingly, the myristoylation results (Figure 6Aand 6B, lanes 5 and 6) strongly suggested that α i2 and sα i2 are translated equally well, as evidenced by similar levels of radiolabeled myristate incorporation during the labeling period, but sα i2 is more rapidly degraded, as seen by reduced steady-state levels of immunoprecipitated protein.
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg cdk2 antibody in 300 μl IP buffer [50 mM Tris (pH 7.5), 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, and 1% NP-40] and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.
Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.