Words similar to immunoprecipitated
Example sentences for: immunoprecipitated
How can you use “immunoprecipitated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Equal amounts (200 μg) of protein from each sample were immunoprecipitated with 0.5 μg Cdk2 antibody in 300 μl IP buffer (50 mM Tris, pH 7.5; 5 mM EDTA, 20 mM glycerol, 150 mM NaCl, 1% NP-40) and 25 μl protein A/G agarose beads (Santa Cruz Biotechnology) on an orbital shaker overnight at 4°C.
Lysates were immunoprecipitated with 3 μl of whole 369 antibody serum and 30 μl of protein A beads.
Upon addition of 10 mM DTT to cell lysates, mAb 6B12 immunoprecipitated only a 85 kD protein band, consistent with specific recognition of the CD98 heavy chain.
Lysates were immunoprecipitated with minor alterations to previous protocols [ 8 ] . Specifically, the IP buffer contained only 50 mM NaCl and incubation with the primary antibody was performed overnight at 4°C.
FLAG-DEK was immunoprecipitated using anti-FLAG resin (Sigma-Aldrich, St Louis, MO, USA) in accordance with the manufacturer's instructions and was eluted by competition with peptide containing three FLAG recognition epitopes.
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