Example sentences for: immunoperoxidase

How can you use “immunoperoxidase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • At regular intervals, the slides were rinsed in PBS, fixed in ice-cold 4% paraformaldehyde for 20 min, washed in PBS, stained with anti-BMPR antibodies, and visualized by the ABC immunoperoxidase method described above.

  • Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

  • The slides were dried ~2 h at room temperature, fixed 5 min in acetone, dried 30 min, and stored at -80°C until immunoperoxidase staining was performed.

  • Immunostaining was performed on frozen sections with FITC- and Cy3-labeled secondary antibodies (Jackson Laboratories, USA), or on paraffin-embedded sections with immunoperoxidase, as described earlier [5].

  • The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).


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