Words similar to immunoprecipitates
Example sentences for: immunoprecipitates
How can you use “immunoprecipitates” in a sentence? Here are some example sentences to help you improve your vocabulary:
Immunoprecipitates were generated from 2 × 10 7cells as described for the ICKA except that the immunoprecipitates were washed once with PBS and resuspended in SDS Sample Buffer.
For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
The immunoprecipitates were pelleted, washed three times with wash buffer (1X TBS pH7.
The lack of most of these endogenous proteasome components in the immunoprecipitates of the two deletion version of HsN3 whose prosequences have been artificially removed via deletions indicates that the prosequence of HsN3 is also important for its successful assembly into the mature 20S proteasome.
Smad1 was also detected in the immunoprecipitates of HsN3 (Fig.