Example sentences for: immunoprecipitation

How can you use “immunoprecipitation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The predominant band in the immunoprecipitation of wild type HsN3 is the prosequence-containing HsN3 (Pro-N3-F), as indicated by its molecular weight and the ability of anti-Flag antibody to detect it in a parallel Western blot.

  • Since equal transfection efficiency was monitored and equal total proteins were used for the immunoprecipitation, the increased signals of pro-N3-F and its associated proteins in Fig.

  • Cell lysates for immunoprecipitation were prepared in IP-LB (25 mM Tris, pH 7.4, 150 mM NaCl, 0.5 % Triton X100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin).

  • After subcellular fractionation with HeLa cells, each fraction underwent immunoprecipitation and subsequent immunoblotting with anti-COP1 antibodies.

  • For immunoprecipitation, 150 μl cell lysates were pre-cleaned by protein A sepharose beads (Pharmacia), incubated with 3 μl of anti-T7 (1 mg/ml) or anti-Flag (3 mg/ml) (M2 monoclonal, IBI, Eastman Kodak) on ice overnight.

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