Words similar to immunoprecipitation

Example sentences for: immunoprecipitation

How can you use “immunoprecipitation” in a sentence? Here are some example sentences to help you improve your vocabulary:

Size fractionation of total cell lysates prior to immunoprecipitation revealed co-elution of Pip1p with Pop1p, Pop2p, Pcu1p, and Psh1p in a high molecular weight complex of approximately 500 kDa, which we refer to as SCF Pop1p-Pop2p(Fig.
Polyclonal rabbit anti-IgM antibodies were used to signal BCL 1 .3B3 through the BCR [ 27 ] . Polyclonal rabbit anti-ovalbumin was used as a negative control for activation and immunoprecipitation experiments.
For immunoprecipitation, the lysates were incubated for 4 h at 4°C with 1 μg of antibody (all from Santa Cruz) pre-adsorbed on Protein A-Sepharose beads (Pharmacia).
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.
A FLAG-tagged DEK adenoviral vector constructed by the University of Michigan Vector Core was used to transduce T98G cells (ATCC) by incubation for 48 hours before harvesting for immunoprecipitation.