Words similar to immunoprecipitation
Example sentences for: immunoprecipitation
How can you use “immunoprecipitation” in a sentence? Here are some example sentences to help you improve your vocabulary:
To investigate this possibility, we carried out immunoprecipitation assays of MCF-7 nuclear extract with a series of antibodies, and immunoblotted with the same series of antibodies.
Western blot and immunoprecipitation analysis
To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
The immunoprecipitation assay followed by western blot analysis was as described previously [ 25].
As predicted, both proteins are recognized by antibody specific for PP1α when performing immunoprecipitation or western blotting.