Example sentences for: immunoprecipitation

How can you use “immunoprecipitation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • However, there was a substantial loss of CDK2 activity as determined by an immunoprecipitation kinase assay (Fig.

  • To confirm the interaction between SNIP1 and Az or HsN3 seen in the yeast two-hybrid system, we transfected COS1 cells with each pair of proteins and tested the interactions by immunoprecipitation assays.

  • For immunoprecipitation, 150 μl cell lysates were pre-cleaned by protein A sepharose beads (Pharmacia), incubated with 3 μl of anti-T7 (1 mg/ml) or anti-Flag (3 mg/ml) (M2 monoclonal, IBI, Eastman Kodak) on ice overnight.

  • Following the immunoprecipitation reaction, samples were analyzed by Western blotting using the opposite antibody, that is, samples that had been immunoprecipitated with anti-SecB were examined with anti-His while those that had been precipitated with anti-His were probed with anti-SecB.

  • To determine whether ubiquitin was conjugated directly to Rab24(D123I), it was necessary to solubilize the protein aggregates in a high-detergent buffer that was incompatible with standard immunoprecipitation methods.


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