Example sentences for: immunocomplexed

How can you use “immunocomplexed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.

  • Having previously reported on the ability of immunocomplexed endogenous PP1 to dephosphorylate pRB [ 20 ] , we followed the same experimental approach.

  • Enzyme samples were immunocomplexed 6His-HA-PP1α.

  • Phosphorylase Phosphatase Activity of Immunocomplexed 6His-HA-PP1α

  • First, immunocomplexed 6His-HA-PP1α was tested for its ability to dephosphorylate phosphorylase a . Phosphorylated on a single serine residue, this substrate is often used to monitor the biochemical activity of PP1 in vitro [ 28 ] . As shown in Figure 5, greater phosphatase activity is detected in immunoprecipitates from induced LLWO2F cell lysates compared to uninduced.

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