Example sentences for: immunoblot

How can you use “immunoblot” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples of the eluted protein were subjected to SDS-PAGE and immunoblot analysis as described previously, using a polyclonal antibody against Rab24 [ 25 ] , or monoclonal antibodies against the His 6 -tag (Sigma) or ubiquitin (Zymed Laboratories).

  • One quarter of each fraction was subjected to SDS-PAGE and immunoblot analysis using the 9E10 monoclonal antibody against the myc epitope (Oncogene Research Products, Cambridge, MA), followed by HRP-conjugated goat anti-mouse IgG and ECL detection reagent (Amersham Corp, Arlington Hts.

  • The expression of HA-Ppt1p was detected by immunoblot using the 12CA5 monoclonal anti-HA antibody (Roche Molecular Biochemicals).

  • Figure 5bshows an immunoblot (anti-HA) of total extracts from HA-Smo-expressing cells treated for 2, 5, 8 and 20 hours with vehicle, 35 nM Hh protein or 0.5 μM Hh agonist.

  • SDS-PAGE and Western blot using chemiluminescence-based detection were performed essentially as described [ 15 ] . Immunoprecipitation of PfPP5 complexes followed by immunoblot analysis of the precipitate were carried out essentially as described [ 34 ] .

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