Example sentences for: immunoblotted

How can you use “immunoblotted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protein samples were resolved on a 10% SDS-PAGE and immunoblotted with primary antibody and revealed with horse radish peroxidase (HRP)-conjugated anti-mouse IgM, or anti-rabbit IgG (Jackson Laboratory, Bar Harbor, Maine, United States).

  • Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.

  • To investigate this possibility, we carried out immunoprecipitation assays of MCF-7 nuclear extract with a series of antibodies, and immunoblotted with the same series of antibodies.

  • In cases where discrepant results were obtained between immunoblot and EIA testing, sera were immunoblotted using a more sensitive technique against both intact and apoptotic Jurkat lysates, as previously described [ 18 19 ] using sera diluted 1:5000.

  • NHBEs were treated with increasing concentrations of CSC and whole-cell extracts were immunoblotted for phosphorylated IκB.


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