Example sentences for: immunoblotted

How can you use “immunoblotted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In cases where discrepant results were obtained between immunoblot and EIA testing, sera were immunoblotted using a more sensitive technique against both intact and apoptotic Jurkat lysates, as previously described [ 18 19 ] using sera diluted 1:5000.

  • Thirty micrograms of cytoplasmic lysates or 20 μg nuclear lysates were loaded onto 12% SDS-PAGE gels and immunoblotted using chemiluminescent antibodies.

  • Protein samples were resolved on a 10% SDS-PAGE and immunoblotted with primary antibody and revealed with horse radish peroxidase (HRP)-conjugated anti-mouse IgM, or anti-rabbit IgG (Jackson Laboratory, Bar Harbor, Maine, United States).

  • Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.

  • Figure 6shows Western blot analysis of nuclear extracts from 10 human breast tumor immunoblotted with polyclonal antibodies NMT-4 (top panel), NMT-5 (middle panel) and monoclonal antibody NMT-1 (bottom panel).


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