Words similar to immunoaffinity
Example sentences for: immunoaffinity
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The antigen-induced arthritis model of rheumatoid arthritis in rabbits demonstrates persistent active inflammation for several months after the intra-articular injection of antigen, including hypertrophy and hyperplasia of the synovial lining cells, pannus formation with articular cartilage erosion, and chronic infiltration of synovium by lymphocytes and plasma cells [ 13 ] . In addition, Dijkstra and colleagues [ 15 ] found macrophages in the superficial layer of the synovium, where they might secrete enzymes and oxygen radicals into the joint space, which can lead to cartilage erosion [ 9 ] . The protein levels in lavage fluid from arthritic joints in this model are low enough to allow direct testing by Western blot analysis without immunoaffinity purification, as was needed with the human aspirates.
Because mactinin is a very small fraction of the total protein content (more than 50 μg/10 μl) of the fluid, immunoaffinity purification of mactinin was performed before Western blotting.
Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
In order to detect cell division during putative diapause, we immunostained the embryos with anti-phospho(S10)-acetyl(K14)-Histone H3 (anti-Histone H3) (rabbit immunoaffinity purified IgG, Upstate Group, Inc., MA).
The finding that mactinin is detected by Western blotting of samples run under reducing conditions without immunoaffinity purification seems to confirm this.
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