Example sentences for: immunocomplexes

How can you use “immunocomplexes” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Immunocomplexes were collected on protein A or G beads, washed five times in IP-LB for 5 minutes each, dissolved in 2 × SDS sample buffer, and separated by SDS gel electrophoresis.

  • Similar polyubiquitylated reaction products were detected upon incubation of phosphorylated Rum1p with Pcu1p immunocomplexes, further suggesting that the activity is mediated by SCF Pop1p-Pop2p(data not shown).

  • Fractionation by SDS-polyacrylamide gel electrophoresis and autoradiography of the resulting immunocomplexes from these cells showed crosslinking exclusively to HA-Smo, but with an efficiency of less than 1% (data not shown).

  • Cell extracts were spun at 14,000 rpm in a microcentrifuge and supernatants were incubated for 40-60 minutes with either anti-HA beads (Roche) or anti-v5 antibody (Invitrogen) and Protein A beads (Pierce; Rockford, USA) to form immunocomplexes.

  • Immunocomplexes were collected by binding to protein A or G beads, washed and analyzed by immunoblotting as described [ 33].


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