Words similar to ifnγ
Example sentences for: ifnγ
How can you use “ifnγ” in a sentence? Here are some example sentences to help you improve your vocabulary:
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
Based on these findings, we speculated that the decrease of IP-10/CXCL10 and MIG/CXCL9 led to a decrease in IFNγ by limiting the influx of T cells producing IFNγ at the tumor site.
To identify putative luteolytic factors that initiate CLENDO cell apoptosis, cells were treated with TNFα (50 ng/ml; Upstate Biotechnology, Inc.; Lake Placid, NY), bovine IFNγ (200 IU/ml; generous gift from Dr. Dale Godson, Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada), sFasL (50 ng/ml; Oncogene, LaJolla, CA), mouse Fas activating antibody (Jo2; 500 ng/ml; Pharmingen, San Diego, CA), human Fas activating antibody (CH-11; 500 ng/ml; Upstate Biotechnology, Inc., Lake Placid, NY), PGF
Neutralization of IFNγ caused a significant decrease in both MIG/CXCL9 and IP-10/CXCL10 indicating that these chemokines are largely IFNγ dependent.
An alternative explanation is that residual cytokines such as IFN-inducible T cell α chemoattractant (ITAC/CXCL11) present after in vivo neutralization of IFNγ, MIG, IP-10 may have remaining activity and attract CXCR3 +veT cells.
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